温针对腰神经根受压模型大鼠组织形态的影响

发布时间：2018-03-06 00:11

本文选题：模型　切入点：动物　出处：《中国组织工程研究》2015年49期 　论文类型：期刊论文

【摘要】：背景:80%以上的腰椎间盘突出症早期患者通过非手术治疗可缓解或者治愈。针灸治疗是非手术治疗中很重要的方法之一,且疗效确切。温针是针灸疗法中很重要的一种方法,具有针刺促使气血调和、通经活络以及艾灸调和气血、舒筋通络、解瘀止痛的双重作用。目的:观察温针对大鼠腰神经根受压模型组织形态的影响。方法:纳入60只SD大鼠,平均分为5组,分别为正常组、模型组、美洛昔康组、针刺组和温针组,每组12只。通过将特制的硅胶片放置在右侧L_5神经根出硬膜囊处,从而对大鼠的腰神经根造成压迫,建立大鼠腰神经根受压模型。正常组不予造模,不予治疗;模型组仅造模,不予治疗;美洛昔康组造模后予以美洛昔康治疗2周;针刺组造模后予以针刺治疗2周;温针组造模后予以温针治疗2周。造模2周后取材,采用苏木精-伊红染色及光镜观察各组大鼠受压腰神经根组织形态变化。结果与结论:动物造模后2周,经病理切片观察神经损伤情况,确认腰神经根受压模型大鼠造模成功。温针组的神经纤维及背根节周围组织炎症反应改善较美洛昔康组和针刺组明显。提示温针比美洛昔康和针刺能更有效的改善大鼠受损神经根组织结构,降低大鼠腰神经根受压模型的炎性反应。
[Abstract]:Background: more than 80% of patients with early lumbar disc herniation can be relieved or cured by non-surgical treatment. Acupuncture is one of the most important methods in non-operative treatment, and the curative effect is definite. Warm needle is an important method in acupuncture therapy. It has the dual effects of acupuncture on promoting qi and blood harmony, meridian activating collaterals and moxibustion to harmonize qi and blood, soothing tendons and unblocking collaterals and relieving stasis and relieving pain. Objective: to observe the effect of temperature on the tissue morphology of lumbar nerve root compression model in rats. Methods: 60 SD rats were included in the study. The rats were divided into 5 groups: normal group, model group, meloxicam group, acupuncture group and warm acupuncture group, 12 rats in each group. The model of lumbar nerve root compression was established in rats. The normal group was not treated, the model group was only established without treatment, the model group was treated with meloxicam for 2 weeks after modeling, the acupuncture group was treated with acupuncture for 2 weeks after making the model, and the control group was treated with meloxicam for 2 weeks. The rats in the warm acupuncture group were treated with warm acupuncture for 2 weeks. After 2 weeks of model making, the rats were stained with hematoxylin eosin and light microscope to observe the morphological changes of the lumbar nerve root under pressure. Results and conclusion: two weeks after the establishment of the model, the rat model was made. The nerve injury was observed by pathological section. It was confirmed that the model of lumbar nerve root compression in rats was successful. The inflammatory response of nerve fibers and peridorsal root ganglion in warm acupuncture group was significantly improved than that in meloxicam group and acupuncture group, which suggested that warm acupuncture was more effective than meloxicam and acupuncture. The injured nerve root tissue structure, The inflammatory response of rat lumbar nerve root compression model was decreased.
【作者单位】：上海市第六人民医院东院针推伤科;上海交通大学附属第六人民医院针推伤科;
【基金】：上海市科委资助课题(04419723):从细胞因子调控探讨温和灸对大鼠腰神经根受压模型的作用机制~~
【分类号】：R245;R-332

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