改良的光化学法血栓模型的建立及消栓通络汤对血栓形成的影响

发布时间：2018-03-07 20:04

本文选题：血栓　切入点：光化学法　出处：《辽宁中医药大学》2008年硕士论文　论文类型：学位论文

【摘要】： 目的:建立一种以光化学反应为基础的改良的光化学法动物血栓模型;基于已经建立起来的改良的光化学法动物血栓模型来探讨消栓通络汤对改良的光化学法动物血栓模型的作用;对治疗血栓性疾病的临床验方消栓通络汤的处方结构进行优化研究。方法:小鼠经尾静脉注射给予孟加拉红,将小鼠耳部平铺于生物机能实验系统和微循环观测系统的显微镜下,用波长为490nm的冷光源照射小鼠耳廓目标血管,观察并记录小鼠耳廓目标血管的血流状态,以不同时间点血流速度及血流完全停止的时间作为评价血栓形成的指标;通过对小鼠一个月的灌胃给药后,用改良的光化学法对小鼠进行造模,观察消栓通络汤组及阿司匹林组之间小鼠耳廓血管不同时间点的血流速度变化、血流停止时间、血小板数变化的差异作为评价消栓通络汤对改良的光化学法动物血栓模型影响的指标;运用均匀设计改良法,以小鼠不同时间点的血流速度变化、血流停止时间、血小板数变化的药效学指标为评价指标,进行处方最佳配伍和最佳剂量配比的比较研究。结果:孟加拉红组在冷光源照射5min时,小鼠耳廓血管血流速度明显减慢,照射到25min时,血管血流完全停止;消栓通络汤组冷光源照射5min后,血流速度明显减慢,照射到25min时,高剂量组、中剂量组血流未停止,低剂量组的血流完全停止,随着剂量的增加,对血小板的聚集的抑制作用增强;以均匀设计法得出了消栓通络汤最佳配伍及最佳剂量配比的理论值,并经验证确认,提取物10(由水蛭3g,当归27g,地龙24g,川芎21g,怀牛膝15g,石菖蒲9g组成)和理论4组(由水蛭3g,当归30g,地龙30g,川芎25g,怀牛膝20g组成)药效最佳,因理论4组药味少1种,故可推荐用于后续新药开发研究。结论:490nm冷光源照射已经注射孟加拉红的小鼠耳廓血管,能够造成小鼠耳廓血管的血流速度减慢,最终血流停止,可以建立以光化学反应为基础的动物血栓模型;消栓通络汤延缓改良的光化学动物血栓模型的血流速度减慢的速度、延长血流停止时间、抑制血小板聚集,具有一定的抗血栓形成作用;本实验研究方法将拆方分析与最佳剂量配比研究结合起来,运用于处方优化具有切实可行性。
[Abstract]:Objective: to establish a modified photochemical animal thrombus model based on photochemical reaction. Based on the established animal thrombus model, the effect of Xiaoshuantongluo decoction on the modified photochemical animal thrombus model was studied. The prescription structure of Xiaoshuantongluo decoction, a clinical prescription for treating thrombotic diseases, was optimized. Methods: the mice were injected with Bengal red through tail vein. The ears of mice were laminated under the microscope of biological function test system and microcirculation observation system. The target blood vessels of mouse auricle were irradiated with a cold light source of 490nm, and the blood flow status of the target vessels of mouse auricle was observed and recorded. The blood flow velocity and the time when the blood flow stopped completely at different time points were taken as the indexes to evaluate thrombosis, and the mice were modeled by modified photochemical method after one month of intragastric administration. To observe the changes of blood flow velocity and blood flow stop time of auricle blood vessel of mice at different time points between the group of Xiao Shuan Tongluo decoction and the group of aspirin. The difference of platelet number was used as an index to evaluate the effect of Xiaoxuan Tongluo decoction on the modified photochemical animal thrombus model, and the uniform design method was used to change the blood flow velocity and stop time of blood flow at different time points in mice. The pharmacodynamics index of platelet number change was used as the evaluation index. The best prescription compatibility and the best dose ratio were compared. Results: the blood flow velocity of auricle blood vessel of mice in Bengal red group was significantly decreased when exposed to cold light source for 5 min, and at 25 min after exposure to Bengal red group. The blood flow of the blood vessel stopped completely in the Xiaoshuantongluo decoction group, and the blood flow velocity of the cold light source in the Xiaoshuantongluo decoction group was obviously slowed down after 5 min irradiation. At 25 min, the blood flow in the high dose group and the middle dose group did not stop, and the blood flow in the low dose group stopped completely with the increase of the dose. The inhibitory effect on platelet aggregation was enhanced, and the theoretical values of the best compatibility and the best dosage ratio of Xiaoxuan Tongluo decoction were obtained by uniform design, and confirmed by verification, Extract 10 (composed of Hirudo 3g, Angelica 27g, Dilong 24g, Ligusticum chuanxiong 21g, Radix bidentata 15g, Acorus calamus 9g) and theoretical group 4 (composed of leeches 3g, angelica sinensis 30g, Dilong 30g, Chuanxiong 25g, Achyranthes bidentata 20g) had the best effect, because the theoretical 4 groups had one less flavor, Conclusion irradiation of auricle vessels injected with Bengal red can slow down the blood flow velocity of the auricle vessels in mice and eventually stop the blood flow. The animal thrombus model based on photochemical reaction can be established, and Xiaoshuantongluo decoction can slow down the blood flow speed, prolong the blood flow stop time and inhibit platelet aggregation in the modified photochemical animal thrombus model. The experimental method combines the disassembly analysis with the optimal dose ratio study, and it is feasible to apply to the optimization of prescription.
【学位授予单位】：辽宁中医药大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R-332;R285.5

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