缺氧对C57小鼠脑胶质瘤模型肿瘤形成及生存期的影响

发布时间：2018-03-08 11:39

本文选题：缺氧　切入点：胶质瘤　出处：《第三军医大学学报》2015年07期 　论文类型：期刊论文

【摘要】：目的探讨缺氧对C57小鼠胶质瘤肿瘤形成及生存期的影响。方法 1培养GL261胶质瘤细胞系,转染荧光素酶基因,制备GL261-Luc细胞。242只清洁健康级雌性C57小鼠[6周龄,体质量(17.5±2.3)g]脑内注射GL261-Luc细胞荷瘤,分成常氧饲养组(21%O2)和低氧饲养组(10%O2),每组21只,其中6只分别于7、14、21 d后行活体成像图像采集以观察小鼠肿瘤形成能力;另外15只用于生存期观察,时限30 d。3取材并HE染色观察肿瘤形成情况,免疫组化GFAP鉴定胶质瘤。结果活体成像显示缺氧饲养组小鼠有明显肿瘤形成,而常氧饲养组无肿瘤形成,计算颅内荷瘤细胞病毒活性并统计分析得缺氧饲养组肿瘤形成能力强于常氧饲养组(P0.05);生存期分析显示缺氧饲养组小鼠平均生存期为22.5 d,生存率明显低于常氧饲养组(P0.01);脑组织大体标本和HE染色发现缺氧组小鼠共18只有脑肿瘤形成,常氧组仅2只形成肿瘤,免疫组化GFAP胶质瘤鉴定示肿瘤内高表达。结论GL261胶质瘤细胞系C57小鼠成瘤模型缺氧饲养后肿瘤形成能力明显强于常氧饲养组,且生存率较常氧组更低,提示缺氧可以增加肿瘤恶性。
[Abstract]:Objective to investigate the effect of hypoxia on tumor formation and survival of C57 mouse glioma. Methods 1 GL261 glioma cell line was cultured and luciferase gene was transfected into GL261-Luc cells. GL261-Luc cells (17.5 卤2.3 g) were injected into the brain and divided into two groups: normoxic feeding group (21O _ 2) and hypoxic feeding group (10O _ 2), 21 rats in each group. Six of them were collected in vivo to observe the tumorigenesis ability of mice after day 1421, and the other 15 mice were used for survival observation. At 30 days, the tumor formation was observed by HE staining, and the glioma was identified by immunohistochemical GFAP. Results the in vivo imaging showed that there was significant tumor formation in mice fed with hypoxia, but there was no tumor formation in normoxic feeding group. The viral activity of intracranial tumor-bearing cells was calculated and statistical analysis showed that the tumor forming ability of hypoxic feeding group was stronger than that of normoxic feeding group P0.05.The survival time analysis showed that the average survival time of hypoxic mice was 22.5 days, and the survival rate was significantly lower than that of normoxic feeding group. Brain tissue samples and HE staining showed that 18 mice in hypoxia group had only brain tumors. Only 2 tumors were formed in normoxic group, and high expression of tumor was detected by immunohistochemical GFAP glioma identification. Conclusion the tumor forming ability of GL261 glioma cell line C57 mice after hypoxia feeding is significantly stronger than that of normoxic feeding group, and the survival rate is lower than that of normal oxygen group. The results suggest that hypoxia can increase the malignancy of tumor.
【作者单位】：第三军医大学西南医院神经外科全军神经外科研究所全军神经创伤防治重点实验室;
【基金】：国家自然科学基金(81272784)~~
【分类号】：R739.41;R-332

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