氧化低密度脂蛋白对THP-1巨噬细胞吞噬和分泌功能的影响

发布时间：2018-03-10 16:50

本文选题：动脉粥样硬化　切入点：巨噬细胞　出处：《现代生物医学进展》2015年04期 　论文类型：期刊论文

【摘要】：目的:探讨氧化低密度脂蛋白(oxidized low density lipoprotein,ox-LDL)对巨噬细胞源性泡沫细胞吞噬功能和炎症相关因子分泌功能的影响。方法:利用佛波酯(phorbol ester,PMA)诱导THP-1细胞分化形成巨噬细胞,之后采用ox-LDL处理48小时后,诱导其形成泡沫细胞。利用中性红吞噬实验,分析泡沫细胞形成前后吞噬功能的变化;通过ELISA法,检测细胞培养上清中肿瘤坏死因子α(tumor necrosis factorα,TNF-α)含量,观察ox-LDL对THP-1巨噬细胞功能的影响。结果:细胞形态学结果表明,我们成功利用ox-LDL诱导THP-1巨噬细胞形成泡沫细胞;进一步发现ox-LDL诱导THP-1巨噬细胞表面的清道夫受体CD36表达升高,并促进细胞吞噬功能增加,进一步促进细胞内胆固醇含量显著升高(P0.05);同时,ox-LDL能够刺激巨噬细胞大量分泌TNF-α(P0.05)。结论:ox-LDL通过增强清道夫受体CD36表达,提高巨噬细胞的吞噬功能,引起大量胆固醇聚集,产生细胞毒性损伤,并促进TNF-α炎性因子的大量分泌。
[Abstract]:Objective: To investigate the effects of oxidized low density lipoprotein (oxidized low density lipoprotein, ox-LDL) related factors affecting phagocytic function and inflammatory secretion of macrophage derived foam cells. Methods: using phorbol-12-myristate-13-acetate (phorbol ester PMA) THP-1 cell differentiation induced by formation of macrophage cells, after treated with ox-LDL for 48 hours, induced the formation of foam cells. Using neutral red phagocytosis test, analysis of the formation of foam cells before and after phagocytosis; by ELISA method, the detection of tumor necrosis factor alpha cell culture supernatant (tumor necrosis factor TNF- alpha, alpha) content, the effect of ox-LDL on function of THP-1 macrophage cell morphology. Results: the results showed that we successfully induced by ox-LDL THP-1 macrophage foam cell formation; we found that ox-LDL induced THP-1 macrophage scavenger receptor CD36 expression increased, and promote phagocytosis To further promote the function to increase the intracellular cholesterol content was significantly increased (P0.05); at the same time, ox-LDL can stimulate the secretion of macrophage TNF- alpha (P0.05). Conclusion: ox-LDL can enhance the expression of scavenger receptor CD36, improve the phagocytic function of macrophage, causing a lot of cholesterol accumulation, produce cytotoxic injury and promote the secretion of inflammatory TNF- alpha factor.

【作者单位】：第四军医大学第一附属医院心内科;第四军医大学基础部病理科;
【基金】：国家自然科学基金项目(81101711)
【分类号】：R392

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