嗜酸乳杆菌和酪酸梭菌协同抑制痢疾杆菌的活性研究

发布时间：2018-03-11 04:42

本文选题：嗜酸乳杆菌　切入点：酪酸梭菌　出处：《中国药科大学学报》2015年01期 　论文类型：期刊论文

【摘要】：以嗜酸乳杆菌和酪酸梭菌为研究对象,用琼脂扩散法检测其纯培养和共培养后的发酵液对福氏痢疾杆菌的体外抑菌活性。结果表明,嗜酸乳杆菌和酪酸梭菌共培养液对福氏痢疾杆菌具有良好的协同抑制作用,其共培养液抑菌作用较嗜酸乳杆菌和酪酸梭菌纯培养,其抑菌作用分别增强了17.2%和22.4%。同时,嗜酸乳杆菌和酪酸梭菌存在共生关系,共培养后生物量可达4.27 g/L(干重),较嗜酸乳杆菌和酪酸梭菌纯培养生物量分别提高6.0%和30.6%,且共生可使产酸增多,从而达到协同抑菌效果。对福氏痢疾杆菌黏附人结肠腺癌Caco-2细胞的研究表明,嗜酸乳杆菌和酪酸梭菌能协同抑制福氏痢疾杆菌对Caco-2细胞的黏附,Caco-2与混合益生菌、嗜酸乳杆菌和酪酸梭菌共孵育的组别,其福氏痢疾杆菌的相对黏附率分别降低了84.2%,81.2%和75.8%,相对应的Caco-2细胞的活力分别提高了22.9%,12.5%和11.5%。本研究首次发现两种益生菌嗜酸乳杆菌和酪酸梭菌具有共生关系,并且在体外能够协同抑制肠道致病菌福氏痢疾杆菌的生长和黏附,对于由福氏痢疾杆菌引起的细菌性痢疾治疗具有临床应用前景。
[Abstract]:The inhibitory activity of Lactobacillus acidophilus and Clostridium caseinate in vitro against Shigella flexneri was detected by Agar diffusion method. The co-culture medium of Lactobacillus acidophilus and Clostridium caseinate had a good synergistic inhibitory effect on Shigella flexneri. The bacteriostatic effect of the co-culture medium was 17.2% and 22.4respectively higher than that of Lactobacillus acidophilus and Clostridium casein. There was a symbiotic relationship between Lactobacillus acidophilus and Clostridium caseinate, and the biomass after co-culture was 4.27 g / L (dry weight, 6.0% and 30.6 higher than that of Lactobacillus acidophilus and Clostridium caseinate respectively), and acid-producing was increased by symbiosis. The study on the adhesion of Shigella flexneri to human colon adenocarcinoma Caco-2 cells showed that Lactobacillus acidophilus and Clostridium caseinate could synergistically inhibit the adhesion of Shigella flexneri to Caco-2 cells and mixed probiotics. A group of Lactobacillus acidophilus and Clostridium caseinate, The relative adhesion rate of Shigella flexneri decreased by 84.2% and 75.8%, respectively, and the activity of corresponding Caco-2 cells increased by 22.9% and 11.55.The two probiotics, Lactobacillus acidophilus and Clostridium caseoides, were found to have symbiotic relationship for the first time. In vitro, it can inhibit the growth and adhesion of Shigella flexneri, which has the prospect of clinical application in the treatment of bacillary dysentery caused by Shigella flexneri.
【作者单位】：中国药科大学生命科学与技术学院;
【基金】：中央高校基本科研业务费专项资金资助项目(No.ZL2014SK0035) 江苏高校优势学科建设工程资助项目~~
【分类号】：R378

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