免疫色谱法从液体培养物中快速鉴定结核分枝杆菌复合群的效果评价

发布时间：2018-03-15 02:26

本文选题：结核分枝杆菌复合群　切入点：MPB蛋白　出处：《环境与职业医学》2015年10期 　论文类型：期刊论文

【摘要】：[目的]评价两种免疫色谱法试剂盒[结核分枝杆菌复合群鉴定试剂盒、结核分枝杆菌抗原检测试剂盒(胶体金法)]对分枝杆菌诊断系统(BACTEC MGIT 960 System)阳性培养物快速鉴定结核分枝杆菌复合群(MTBC)的临床价值。[方法]对15株分枝杆菌标准菌株及418例BACTEC MGIT 960阳性培养物采用两种免疫色谱法试剂盒和传统生化法鉴定结核分枝杆菌复合群和非结核分枝杆菌。鉴定结果不一致的菌株,应用16S r RNA基因序列法进行检测。[结果]以传统生化法为金标准,胶体金法鉴定结核分枝杆菌复合群的灵敏度为98.4%(253/257),特异度为99.4%(160/161),阳性预期值为99.6%(253/254),阴性预期值为97.6%(160/164);MGIT TBc ID试剂盒鉴定结核分枝杆菌复合群的灵敏度为98.4%(253/257),特异度为100%(161/161),阳性预期值为100%(253/253),阴性预期值为97.6%(161/165)。与传统生化法比较,2种方法的一致性分别是Kappa值0.975、0.980,两种方法的一致性均较好。5株鉴定结果不一致的菌株应用16S r RNA基因序列法检测结果分别为4株MTBC,1株诺卡氏菌。[结论]胶体金法能快速鉴定结核分枝杆菌复合群,可用于BACTEC MGIT 960培养系统分枝杆菌培养阳性物的鉴定,适合在临床实验室开展。MGIT TBc ID试剂盒检测的准确性高,更适合应用在突发公共卫生检测。
[Abstract]:[objective] to evaluate two immunochromatographic kits [Mycobacterium tuberculosis complex identification kit]. The clinical value of Mycobacterium tuberculosis antigen detection kit (colloidal gold assay) for rapid identification of mycobacterium tuberculosis complex MTBCc by positive culture of BACTEC MGIT 960 system. [methods] 15 strains of mycobacterium standard bacteria were tested. Two immunochromatographic kits and traditional biochemistry methods were used to identify mycobacterium tuberculosis complex and non-tuberculosis mycobacterium strains in 418 BACTEC MGIT 960 positive cultures. The 16s r RNA gene sequence method was used to detect the results. [results] the traditional biochemistry method was used as the gold standard. The sensitivity of colloidal gold method for the identification of mycobacterium tuberculosis complex was 98.4 / 253 / 257, the specificity was 99.4 / 160 / 161, the positive expected value was 99.6 / 253 / 254, and the negative predictive value was 97.66 / 164MGIT TBc ID kit for the identification of mycobacterium tuberculosis complex. The sensitivity of the kit for identification of mycobacterium tuberculosis complex was 98.44 / 253257g, and the specificity was 100 161p161g / 161a. The period value was 100 / 253 / 253G, the negative expected value was 97.6 / 1650.Compared with the traditional biochemistry method, the consistency of the two methods was 0.975- 0.980, respectively. The consistency of the two methods was better than that of the other methods. 5 strains with different identification results were detected by 16s r RNA gene sequencing method. There were 4 strains of MTBCX and 1 strain of Nocardia. [conclusion] Colloidal gold method can be used to identify mycobacterium tuberculosis complex rapidly. It can be used for the identification of mycobacteria positive in BACTEC MGIT 960 culture system. It is suitable for carrying out the detection of .MGIT TBc ID kit in clinical laboratory with high accuracy, and is more suitable for the detection of sudden public health.
【作者单位】：上海市疾病预防控制中心结核病防治科;
【基金】：上海市卫生系统优秀青年人才培养计划(编号:XYQ2011051) 上海市卫生局课题(编号:20114073)
【分类号】：R378.911

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