细胞滚动黏附过程的小波分析方法

发布时间：2018-03-15 11:31

本文选题：细胞滚动黏附　切入点：小波分析　出处：《华南理工大学》2010年硕士论文　论文类型：学位论文

【摘要】： 细胞滚动黏附是血栓形成和炎症反应过程中的重要阶段。揭示细胞滚动黏附的物理过程,对于深入了解白细胞如何在血流剪切作用下到达炎症部位,血小板如何发生黏附和聚集等相关的生理病理过程,以及对抗炎、抗肿瘤、溶解血栓等药物的设计,均具有十分重要的意义。本论文以E-选择素/PSGL-1调控的HL-60细胞黏附为研究对象,以流动腔实验技术和小波分析为手段,研究了细胞滚动黏附过程中的热响应动力学问题。其主要内容包括以下三个方面: 一、通过小波分析研究滚动黏附的物理过程。流动腔实验技术是研究流体环境下细胞-分子作用的重要技术,可获得纳米级别的分子信息,但由于其在实验过程中,会受到细胞的不规则性、流场的局部扰动、实验系统误差等因素的影响,实验结果不可避免地包含了一定的噪声波动。本文通过对细胞运动过程中的噪声的统计分析,确定了对应的小波分析方法,对细胞的运动过程进行了降噪处理,准确识别出了原始信号中细胞黏附和解离的时刻,并根据降噪后的数据结果判断细胞运动过程中的瞬时黏附-解离事件。二、建立细胞自由状态和拴缚状态的热响应动力学模型。通过朗之万方程和能量均分定理,对细胞自由状态的能量特性进行描述,并结合拴缚细胞的振子反应系统建立模型,推导出拴缚细胞在受流体和分子键作用下的能量分布。为了解决由于受到实验条件限制而出现的采样时间间隔不等的问题,本文分析了热响应信号检测的时间尺度效应问题,以方便对实验数据进行统一化分析。三、对细胞运动过程中的噪声来源和能量特性进行分析。通过对细胞运动状态的各物理量进行小波分析,分解其不同运动阶段的细节分量,对不同的变化趋势给出相应的物理解释,建立分子键空间约束和振子系统双效应模型;通过对速度分量进行三层分解,对各种可能的噪声因素进行分离,找到了不同频率噪声可能的来源;分别对X和Y单方向速度进行三层分解,结合总速度分层得到的噪声来源,验证了拴缚细胞的热响应模型中提出的能量分布。本文确立了小波分析方法对流动腔实验数据降噪的有效性,建立并验证了细胞运动过程中自由和拴缚阶段的热响应动力学模型,对细胞滚动黏附问题的进一步研究具有重要的作用。
[Abstract]:Cell rolling adhesion is an important stage in the process of thrombosis and inflammatory reaction. To reveal the physical process of cell rolling adhesion, it is important to understand how white blood cells reach the inflammatory site under the action of blood flow shear. How platelets develop the physiological and pathological processes associated with adhesion and aggregation, as well as the design of drugs such as anti-inflammation, anti-tumor, thrombolysis, etc. In this thesis, the adhesion of HL-60 cells regulated by Eselectin / PSGL-1 was studied, and the flow chamber technique and wavelet analysis were used to study the adhesion of E- selectin / PSGL-1-regulated HL-60 cells. The thermal response kinetics in the process of cell rolling adhesion is studied. The main contents include the following three aspects:. First, the physical process of rolling adhesion is studied by wavelet analysis. The flow chamber experimental technique is an important technique to study cell-molecular interaction in fluid environment. It will be influenced by the irregularity of cell, the local disturbance of flow field, the error of experimental system and so on. The experimental results inevitably contain certain noise fluctuation. The corresponding wavelet analysis method is determined, and the noise reduction of the cell motion process is carried out, and the time of cell adhesion and dissociation in the original signal is accurately identified. The transient adhesion-dissociation events in the course of cell motion were judged according to the data after noise reduction. Secondly, the dynamic models of thermal response of free state and binding state of cells are established. The energy characteristics of free state of cells are described by Langevin equation and energy equalization theorem, and the model of the vibrator reaction system of the tied cells is established. In order to solve the problem of unequal sampling time interval due to the limitation of experimental conditions, the time-scale effect of thermal response signal detection is analyzed in this paper. In order to facilitate the unified analysis of experimental data. Third, the source of noise and energy characteristics during cell motion are analyzed. By wavelet analysis of the physical quantities in the state of cell motion, the detailed components in different motion stages are decomposed. The corresponding physical explanation of different trends is given, and the double effect model of molecular bond space constraint and vibration subsystem is established, and various possible noise factors are separated by three-layer decomposition of velocity component. The possible sources of noise at different frequencies are found and the energy distribution proposed in the thermal response model of the bundled cells is verified by decomposing X and Y unidirectional velocities into three layers and combining the noise sources obtained from the total velocity stratification. In this paper, the validity of wavelet analysis to the noise reduction of flow chamber experimental data is established, and the dynamic model of thermal response in the free and bundled stages of cell motion is established and verified. It is important to further study the problem of cell rolling adhesion.
【学位授予单位】：华南理工大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R364

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