母婴分离对小鼠行为学及胶质纤维酸性蛋白表达的影响

发布时间：2018-03-16 05:10

本文选题：母婴分离　切入点：行为学　出处：《广东医学》2015年15期 　论文类型：期刊论文

【摘要】：目的探讨母婴分离对小鼠行为学及胶质纤维酸性蛋白(GFAP)表达的影响。方法 9只C57BL/6N孕小鼠随机分为3组:对照组、MS15组和MS180组,每组F0代母鼠3只;所产F1代小鼠,分别于出生第2~21天进行母婴分离,母婴分离时先将F0代母鼠隔离至单独鼠笼,将F1代子鼠移至孵育箱中。MS15组母婴分离时间9:00—9:15 am,MS180组分离时间9:00—12:00 am,对照组不分离。采用旷场实验评价母婴分离对小鼠自发活动的影响,强迫游泳和强迫悬尾实验评价母婴分离对小鼠不动时间的影响,Western blot免疫印迹法检测GFAP蛋白表达,Real-time PCR检测GFAP mRNA表达。结果旷场实验中各组小鼠活动总路径差异无统计学意义(P0.05)。与对照组及MS15组比较,MS180组在强迫游泳实验、强迫悬尾实验中,小鼠不动时间显著增加(P0.01);GFAP蛋白及mRNA表达显著升高(P0.01)。结论母婴分离对小鼠行为有显著影响,小鼠出现抑郁样行为,GFAP蛋白和mRNA水平都升高。
[Abstract]:Objective to investigate the effect of maternal and infant isolation on behavior and expression of glial fibrillary acidic protein (GFAP) in mice. Methods Nine pregnant mice of C57BL / 6N were randomly divided into three groups: control group (MS15) and MS180 group (3 female mice of each F 0 generation), F1 generation mice. The mother and child were separated from mother to child on the 2nd day of birth, and the F 0 generation mother mouse was isolated into a single cage when the mother and child were separated from each other. The F1 offspring were transferred to the incubator. The mother and child separation time of MS15 group was 9: 00-9: 15 amMS180 group, while the control group was not separated. Open field experiment was used to evaluate the effect of maternal and infant separation on spontaneous activity of mice. Effects of forced swimming and forced tail suspension on the immobility time of mice were evaluated. Western blot Western blotting was used to detect the expression of GFAP protein and Real-time PCR to detect the expression of GFAP mRNA. Results in the open field experiment, there was no significant difference in the total activity path of mice in each group. Compared with control group and MS15 group, MS180 group was in forced swimming experiment. In the forced tail suspension test, the immobility time of mice increased significantly, and the expression of P0.01GFAP protein and mRNA increased significantly. Conclusion Maternal and infant separation has a significant effect on the behavior of mice, and the levels of GFAP protein and mRNA in mice with depressive behavior are increased.
【作者单位】：南京中医药大学护理学院;南通大学基础医学院;南京中医药大学基础医学院;
【基金】：国家自然科学基金资助项目(编号:81473791) 江苏省优势学科建设工程项目(编号:YSHL0201-19) 南京中医药大学青年自然科学基金资助项目(编号:13XZR32)
【分类号】：R-332

【参考文献】