健康人和HIV感染者非特异性IgG，IgG1-4及gp41特异性IgG1的研究

发布时间：2018-03-16 15:03

  本文选题：艾滋病病毒　切入点：免疫球蛋白G亚型　出处：《中国疾病预防控制中心》2010年硕士论文　论文类型：学位论文

【摘要】： 研究背景免疫球蛋白G (immunoglobulin G, IgG)有四个亚型[1]。IgG1-4水平的变化往往意味着机体免疫力受损或者受到病原微生物的感染[2-5],因此检测IgG1-4水平可以用来评估HIV感染者免疫水平和疾病进程[6]。一些国家和地区的研究结果证明,IgGl-4表达水平在不同人种间存在差异[7]；对于HIV感染者的相关研究显示,HIV感染者拥有较高的IgG1和IgG3,IgG2低于对照人群,IgG4则无显著性差异[8]。我国在该领的研究非常有限。 本研究以北京市健康成人和贵州、重庆两地的HIV感染者为研究对象,开展了健康人IgG、IgG1-4, HIV感染者IgG, IgG1-4和gp41特异性IgG1的研究。该研究对于了解我国健康人和HIV感染者免疫水平、特异性免疫反应,发展相关的免疫学检测方法具有重要意义。 研究目的1、研究测定北京市健康成人IgG. IgG1-4水平,为其他研究提供基础资料。2、研究测定HIV感染者IgG、IgG1-4水平,并对不同感染途径人群的结果进行比较研究,探讨将其作为免疫学评价的指标。3、研究HIV感染者gp41特异性IgG1的产生和变化,探讨作为潜在新近感染检测指标的可能性。 材料和方法收集健康人群血浆315份；HIV阳性IDU人群血浆222份；HIV阳性MSM人群血浆115份；HIV阳性队列样本80份。用夹心ELISA法定量检测血浆中IgG水平；用竞争ELISA方法定量检测血浆中IgG1-4水平；用间接ELISA法定量检测HIV阳性人群血浆中gp41特异性IgG1。对实验结果采用非参数秩和检验进行统计分析[9]。 研究结果1、北京市健康成人IgG, IgG1-4水平分别为：4.83mg/ml、7.84 mg/ml、3.55 mg/ml、0.34 mg/ml和0.23 mg/ml; IgG、IgG4在性别间有差异；IgGl-3有随着年龄增长而下降的趋势。2、HIV阳性人群IgG1和IgG3均有随着CD4细胞计数的升高而增设的趋势；HIV阳性人群IgG与健康成人相比无差异；HIV阳性IDU人群IgG2与CD4细胞计数之间的关系与MSM人群截然相反；HIV阳性MSM人群IgG4与健康成人相比无统计学差异。HIV阳性IDU、MSM人群IgG IgG1-4存在统计学差异。 3、HIV阳性人群gp41特异性IgG1随着CD4细胞计数的下降有降低的趋势,但变化趋势不明显。HIV阳性IDU、MSM人群之间gp41特异性IgG1有统计学差异。4、gp41特异性IgG1随着阳转时间的延长有升高的趋势,但升高的幅度不明显；168天以内感染者与168以上感染者gp41特异性IgG1浓度无统计学差异。 研究结论1、北京市健康成人IgG、IgG1-4水平分别为：4.83mg/ml,7.84 mg/ml、3.55 mg/ml、0.34 mg/ml和0.23 mg/ml。2、可以尝试用IgG1和Ig3做为评估HIV感染者免疫状况或疾病进程的指标；IgG、IgG2和IgG4不宜作为评价HIV感染者免疫状态的指标。不同传播途径对IgG、IgG1-4的表达水平可能造成影响。3、不宜用gp41特异性IgG1作为评价HIV感染者免疫状况或病痛进程的指标。不同传播途径对gp41特异性IgG1可能造成影响。4、不宜用gp41特异性IgG1作为识别新近和既往长期感染的检测指标。
[Abstract]:Protein research background of immunoglobulin G (immunoglobulin G IgG) change four subtype [1].IgG1-4 levels often mean immunity damaged or infected [2-5] of pathogenic microorganisms, so to detect the level of IgG1-4 can be used to study the evaluation of HIV infection immunity and disease progression of [6]. in some countries and regions the results show that the expression level of IgGl-4 in the different people are different [7] species; for the research of HIV infection, HIV infection had higher IgG1 and IgG3, IgG2 lower than the control group IgG4, there was no significant difference of [8]. in our country is very limited in the research area.
In this study, healthy adults in Beijing city and Guizhou, Chongqing two HIV infection as the research object, the health of human IgG, IgG1-4, HIV and IgG1-4 of IgG infection, gp41 specific IgG1. The study to understand the immune level of healthy people in China and HIV infection, specific immune response is important the significance of the development of immunological detection methods.
Objective: 1, determination of IgG1-4 level of healthy adults in Beijing city IgG..2, to provide basic data for other studies, the researchers measured IgG HIV infection, IgG1-4 level, and the population of different routes of infection are compared with the results of the study, explore the immunological evaluation index of.3 as the emergence and change of gp41 specific IgG1 research to explore the possibility of HIV infection, as a potential new infections detection index.
Materials and methods: We collected samples of 315 healthy population; HIV positive among IDU samples of 222 HIV positive MSM patients; 115 plasma samples; HIV positive cohort 80 samples by IgG. The level of quantitative sandwich ELISA detection in plasma; by competitive ELISA method for quantitative detection of plasma IgG1-4 levels in plasma; ELISA method for quantitative detection of HIV positive people gp41 specific IgG1. on experimental results using the nonparametric Wilcoxon test was used for statistical analysis [9].
The results of 1 healthy adults in Beijing City, IgG, IgG1-4 levels were 4.83mg/ml, 7.84 mg/ml, 3.55 mg/ml, 0.34 mg/ml and 0.23 mg/ml; IgG IgG4, there are differences in the different sex; IgGl-3 has decreased with the age growth trend of.2, IgG1 and IgG3 were HIV positive patients with elevated CD4 cell count and additional the trend of HIV; IgG positive people with healthy adults compared with no difference between IgG2 and MSM; the relationship between population HIV positive IDU patients with CD4 cell counts in the opposite; IgG4 HIV positive MSM group no significant difference in the.HIV positive IDU compared with healthy adults, there were significant differences among MSM IgG IgG1-4.
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