脾胃气虚型胃溃疡大鼠病证结合模型的制备

发布时间：2018-03-17 00:28

本文选题：胃溃疡　切入点：脾胃气虚　出处：《山东中医杂志》2015年01期 　论文类型：期刊论文

【摘要】：目的:制备胃溃疡脾胃气虚证的大鼠模型,为证型临床客观化、疗效判定规范化奠定基础。方法 :采用"过食酸味加耗气破气"法制备模型,通过行为学观察和检测血清相关指标检验验证造模是否成功。结果:模型组大鼠便溏、脱肛、消瘦较为严重,反证组有所改善;模型组胃蛋白酶活性明显大于空白组,差异具有高度统计学意义(P0.001),反证组胃蛋白酶活性明显低于模型组,差异具有高度统计学意义(P0.01);模型组MDA含量高于空白组及反证组,但与空白组比较无显著性差异,与反证组有显著性差异(P0.05);模型组胸腺湿重和指数、NO、6-keto-PGF1α含量及SOD活性明显低于空白组,分别具有不同显著性差异(P0.01,P0.05,P0.001,P0.001),反证组该四种指标明显高于模型组,分别具有不同显著性差异(P0.05,P0.05,P0.001,P0.01)。结论 :采用"过食酸味加耗气破气"法制备的脾胃气虚证胃溃疡模型符合临床症状表现,验证胃溃疡"脾胃气虚"型大鼠模型成功。
[Abstract]:Objective: to establish a rat model of spleen and stomach qi deficiency syndrome of gastric ulcer, and to lay a foundation for clinical objectivity and standardization of curative effect. Results: the rats in the model group had loose stools, removed anus, and lost weight, but in the negative group, the pepsin activity in the model group was significantly higher than that in the blank group, and the activity of pepsin in the model group was significantly higher than that in the blank group. The activity of pepsin in the negative group was significantly lower than that in the model group (P 0.01), the MDA content in the model group was higher than that in the blank group and the control group, but there was no significant difference compared with the blank group. There were significant differences between the model group and the negative group (P 0.05), the content of thymus wet weight and the index of no _ (6-keto-PGF _ (1 伪)) and the activity of SOD in the model group were significantly lower than those in the blank group, and there were significant differences between the two groups (P _ (0.01) P _ (0.05) P _ (0.001) P _ (0.001) P _ (0.001) P _ (0.001)), the four indexes in the model group were significantly higher than those in the model group. There were significant differences between them. Conclusion: the gastric ulcer model of spleen and stomach qi deficiency syndrome is in line with the clinical symptom, and it is proved that the gastric ulcer model of "spleen and stomach qi deficiency" is successful.
【作者单位】：山东省中医药研究院;诸城市中医医院;
【基金】：山东中医药科技发展计划(编号:2011-166) 山东省自主创新成果转化重大专项(编号:2012ZHZX1C0405)
【分类号】：R285.5;R-332

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