大鼠腹部异位心脏移植模型的建立

发布时间：2018-03-17 10:44

本文选题：心脏移植　切入点：模型　出处：《昆明医学院》2008年硕士论文　论文类型：学位论文

【摘要】： 目的:建立一种快速、有效的大鼠异位心脏移植模型。方法:以雄性SD大鼠为供体,Wistar大鼠为受体,将供心的升主动脉与受体的腹主动脉行端侧吻合,切除受体的左肾,将供心的肺动脉与受体的左肾静脉以自制聚乙烯管直接插入法连接。形成受体腹主动脉→供体主动脉→冠状动脉→心肌→冠状静脉→右房→右室→肺动脉→受体左肾静脉→下腔静脉的血液循环,开放血管夹,恢复供心血供。术后每日触摸腹部观察移植心脏的搏动情况,移植心脏搏动24 h以上标志移植成功。结果:1.模型建立成功. 35例实验成功32例,成功率91.4%,供心平均缺血时间为(37.8±1.8)~(min)。失败的原因:吻合口出血,血栓形成。 2.病理学及电镜结果移植后,病理提示心脏移植后受体各脏器,移植心脏均发生明显的病理改变。光镜下可见心肌纤维肿胀,部分心肌纤维断裂、紊乱,肌间隙增宽,心肌纤维间可见出血及灶性坏死,微小血管血栓形成。有较多炎性细胞浸润。心肌冠状小动脉管壁肿胀、增厚、官腔变小。电镜显示细胞皱褶、部分断裂,细胞器外溢,细胞核不规则,染色质边集,线粒体嵴模糊、部分消失、线粒体间空泡较多、线粒体内出现空泡及致密颗粒。肌原纤维排列紊乱、均质化,肌节模糊、明暗带模糊;偶见染色质密集,浓缩。结论:本术式操作简单实用,不需手术显微镜,易于推广。
[Abstract]:Objective: to establish a rapid and effective model of heterotopic heart transplantation in rats. Methods: the ascending aorta of the donor heart was anastomosed with the abdominal aorta of the recipient, and the left kidney of the recipient was removed by end-to-side anastomosis with male SD rats as the donor and Wistar rat as the recipient. The donor pulmonary artery is connected to the recipient's left renal vein by direct insertion of a self-made polyethylene tube to form the recipient's abdominal aorta. 鈫扗onor aorta. 鈫扖oronary artery. 鈫扢yocardium. 鈫扖oronary vein. 鈫扲ight atrium. 鈫扲ight ventricle. 鈫扨ulmonary artery. 鈫扲eceptor left renal vein. 鈫払lood circulation of inferior vena cava, opening of blood vessel clamp, restoration of blood supply to heart, daily touching abdomen to observe the beating condition of the transplanted heart after operation, the transplanted heart was successfully transplanted for more than 24 hours. Results 1. The model was successfully established. Thirty-two cases were successful in the experiment, the success rate was 91.4%. The mean ischemic time of donor heart was 37.8 卤1.8 min. The causes of failure were anastomotic bleeding and thrombosis. 2. Pathological and electron microscopic findings. After transplantation, pathology showed that the recipient organs and the transplanted heart had obvious pathological changes. Under light microscope, myocardial fibers were swollen, some myocardial fibers were broken, disordered, and the muscle space widened. Hemorrhage, focal necrosis, microvascular thrombosis, infiltration of inflammatory cells, swelling and thickening of the wall of myocardial coronal arterioles, and small lumen of the coronal arteries were observed in myocardial fibers. Electron microscopy showed that the cells were wrinkled, partially broken, and organelle efflux was observed. The nuclei were irregular, chromatin edge set, mitochondrial ridge blurred, partial disappearance, there were more vacuoles between mitochondria, vacuoles and dense granules appeared in mitochondria, myofibrils were arranged disorderly, homogenized, sarcomere blurred, light and dark zone blurred. Occasionally, chromatin is dense and concentrated. Conclusion: the operation is simple and practical, and it is easy to be popularized without the operation microscope.
【学位授予单位】：昆明医学院
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R654.2;R-332

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