激素性股骨头缺血性坏死模型兔股骨组织中间隙连接蛋白Cx43的表达

发布时间：2018-03-19 22:00

本文选题：组织工程　切入点：股骨头坏死　出处：《中国组织工程研究》2015年18期 　论文类型：期刊论文

【摘要】：背景:激素性股骨头缺血性坏死的发病机制至今尚未完全阐明,间隙连接蛋白Cx43作为骨组织细胞间的主要间隙连接,通过介导骨细胞间信息、能力的正常传递参与调控骨组织细胞生长、分化,代偿性的骨增加或减少,间隙连接蛋白Cx43与激素性股骨头坏死发生发展的关系国内外尚少见报道。目的:通过建立兔激素性股骨头坏死模型,初步探讨间隙连接蛋白Cx43在激素性股骨头坏死中的表达变化。方法:将新西兰大白兔40只随机等分为两组,模型组采用内毒素+激素方法构建激素性股骨头坏死模型,对照组于相同时间点注射等量生理盐水。结果与结论:建模4周后,苏木精-伊红染色结果显示,模型组股骨头软骨下区骨小梁变细,结构紊乱,空骨陷窝明显增多,脂肪细胞增多,髓腔内造血细胞明显减少;对照组股骨头软骨下区骨小梁排列整齐、很少见空骨陷窝,骨髓造血细胞丰富,脂肪细胞较少;免疫组织化学检测显示:Cx43蛋白阳性表达于骨小梁边缘成骨细胞及骨小梁内骨细胞胞浆上及骨髓细胞间质。Western blot检测显示,碱性磷酸酶和Cx43蛋白的表达在模型组中低于对照组(P0.05)。结果证实,在激素性股骨头坏死兔模型组织中Cx43蛋白表达下降,可能是激素性股骨头坏死发生发展的重要环节。
[Abstract]:Background: the pathogenesis of steroid-induced avascular necrosis of the femoral head has not been fully elucidated. Gap junction protein (Cx43), as the main gap junction between bone cells, mediates the information between bone cells. The normal transmission of ability is involved in regulating the growth, differentiation, and compensatory increase or decrease of bone tissue cells. The relationship between gap junction protein (Cx43) and the occurrence and development of steroid-induced osteonecrosis of femoral head is rarely reported at home and abroad. To investigate the expression of gap junction protein Cx43 in steroid-induced femoral head necrosis, 40 New Zealand white rabbits were randomly divided into two groups. The model group used endotoxin hormone to construct steroid-induced femoral head necrosis model. Results and conclusion: after 4 weeks of modeling, the results of hematoxylin-eosin staining showed that the bone trabeculae in the subchondral region of the femoral head of the model group became thin, the structure was disordered, and the empty bone lacunae increased obviously. In the control group, the bone trabeculae in the subchondral area of femoral head were arranged neatly, the empty bone lacunae were rare, the hematopoietic cells in bone marrow were abundant, and the adipocytes were less. Immunohistochemical analysis showed that the positive expression of Cx43 protein in bone trabecular margin osteoblasts and bone trabeculae osteocytes cytoplasm and bone marrow stroma. Western blot detection showed that the expression of Cx43 protein was positive in bone trabecular margin osteoblasts. The expression of alkaline phosphatase and Cx43 protein in the model group was lower than that in the control group (P 0.05). The results showed that the decrease of Cx43 protein expression in the rabbit model of steroid-induced femoral head necrosis might play an important role in the occurrence and development of steroid-induced femoral head necrosis.
【作者单位】：广西医科大学第一附属医院骨关节外科;
【基金】：国家自然科学基金(81460348) 广西教育厅一般项目(2013YB314)~~
【分类号】：R681.8;R-332

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