蝮蛇毒诱导小鼠急性肾损伤及肾组织TNF-α表达的研究

发布时间：2018-03-23 21:38

本文选题：急性肾损伤　切入点：蝮蛇毒　出处：《南华大学》2010年硕士论文

【摘要】： 目的建立蝮蛇毒诱导的小鼠急性肾损伤模型,观察肾脏病理及肾组织TNF-α的表达情况。方法根据蝮蛇毒剂量分为N组(0.00 mg/kg)、L组(0.50 mg/kg)、H组(1.00 mg/kg),每组按时相点又分为3 h、6 h、24 h、48 h四个亚组,96只成年雄性昆明小鼠完全随机分配到各亚组。采取肌注蝮蛇毒方式建立动物模型。在各时相点摘右眼球取血测定肾功能,收集肾组织,制作石蜡标本切片观察:HE染色,光微镜下观察并评分;采用免疫组织化学二步法检测肾组织中TNF-α的表达情况并进行半定量分析。使用SPSS 13.0统计软件进行统计学处理,计量资料采用方差分析,相关性采用直线相关分析。P0.05为有统计学意义。结果 1血清BUN、SCr值:L组(0.50 mg/kg)、H组(1.00 mg/kg)与N组(0.00 mg/kg)比较差别有统计学意义(P0.05)。H组与L组比较差别有统计学意义龴P0.05㖞。N组无变化(P0.05),L组24 h升高48 h下降龴P0.05㖞,H组持续升高龴P0.05㖞。 2 L组(0.50 mg/kg)、H组(1.00 mg/kg)肾脏HE染色:小鼠肾脏均有肾小囊扩大,肾小管变性、萎缩,小管周毛细血管充血。 3光镜下肾损伤病理评分:L组(0.50 mg/kg)、H组(1.00 mg/kg)与N组(0.00 mg/kg)比较差别有统计学意义(P0.05)。H组与L组比较差别有统计学意义龴P0.05㖞。N组无变化(P0.05),L组6 h开始升高48 h下降龴P0.05㖞,H组3 h开始持续升高龴P0.05㖞。 4肾组织TNF-αIHS:L组(0.50 mg/kg)、H组(1.00 mg/kg)与N组(0.00 mg/kg)比较差别有统计学意义(P0.05)。H组与L组比较差别有统计学意义龴P0.05㖞。N组无变化(P0.05),L组6 h开始升高48 h下降龴P0.05㖞,H组3 h开始持续升高龴P0.05㖞。 5相关性分析:BUN与光镜下肾损伤病理评分呈正相关(L组:r=0.793,P0.01;H组:r=0.870,P0.01)。SCr与光镜下肾损伤病理评分呈正相关(L组:r=0.883,P0.01;H组:r=0.891,P0.01)。TNF-αIHS与光镜下肾损伤病理评分呈正相关(L组:r=0.835,P0.01;H组:r=0.953,P0.01)。结论 1肌注0.50 mg/kg和1.00 mg/kg蝮蛇毒都可成功建立小鼠急性肾损伤模型。 2急性肾损伤的严重程度与蝮蛇毒剂量呈正相关,与作用时间存在一定关系。 3肾组织TNF-α的表达与急性肾损伤的严重程度正相关,提示TNF-α可能作为蝮蛇毒诱导小鼠急性肾损伤的重要炎症介质之一。
[Abstract]:Purpose. The acute renal injury model of mice induced by Agkistrodon halys venom was established and the renal pathology and the expression of TNF- 伪 in renal tissue were observed. Method. According to the dosage of Agkistrodon halys Agkistrodon halys, divided into N group (0.50 mg 路kg ~ (-1) 路kg ~ (-1)) and 1. 00 mg 路kg ~ (-1) 路kg ~ (-1) of each group, each group was divided into four subgroups (3 h ~ 6 h ~ 24 h ~ 48 h), 96 adult male Kunming mice were randomly assigned to each subgroup by intramuscular injection of Agkistrodon halys venom. Animal models. Blood was taken from the right eye at different time points to determine renal function. The renal tissues were collected and paraffin sections were made to observe the staining of w: he, and to observe and score under the light microscopes. The expression of TNF- 伪 in renal tissue was detected by immunohistochemical two-step method and semi-quantitative analysis was carried out. Statistical analysis was performed with SPSS 13.0 statistical software, and variance analysis was used to measure the data. Correlation using linear correlation analysis. P0.05 for statistical significance. Results. (1) there was a significant difference in serum BUNN SCR value between group 1. 50 mg / kg and group 1. 00 mg / kg) and group N (P 0. 05 mg / kg). There was a significant difference between group H and group L. There was a significant difference between group H and group L. There was a significant difference between group H and group L. There was a significant difference between group H and group L (P < 0. 05). P0.05? No change in group N, P0.05L group was increased 48 h after 24 h increase and decrease. P0.05? Group H continued to increase? P0.05? . 2HE staining of kidney in group L (0.50 mg 路kg ~ (-1) ~ (-1) mg 路kg ~ (-1)): there were enlarged renal vesicles, tubule degeneration, atrophy and capillary congestion around tubules. 3The pathological score of renal injury under light microscope was 0.50 mg 路kg ~ (-1) mg 路kg ~ (-1) and 1.00 mg 路kg ~ (-1) (P < 0.05). There was a significant difference between group H and group L (P < 0.05). There was a significant difference between group H and group L (P < 0.05). There was a significant difference between group H and group L (P < 0.05). P0.05? Group N showed no change. P0.05L group began to increase and decrease 48 h after 6 hours. P0.05? Group H began to increase continuously at 3 h. P0.05? . (4) there were significant differences in renal tissue between TNF- 伪 IHS:L group (0.50 mg / kg) and N group (1.00 mg / kg). There was significant difference between group H and group L (P 0.05 mg / kg). There was a significant difference between group H and group L (P 0.05 mg / kg). There was a significant difference between group H and group L (P 0.05 mg / kg). P0.05? Group N showed no change. P0.05L group began to increase and decrease 48 h after 6 hours. P0.05? Group H began to increase continuously at 3 h. P0.05? . (5) the correlation between the pathological score of renal injury under light microscope and the weight bun was positive correlation with the pathological score of renal injury under light microscope. There was a positive correlation between the pathological score of renal injury in group 1: 0. 793 and the pathological score of renal injury in group 1: 0. 870 and the pathological score of renal injury under light microscope. There was a positive correlation between the pathological score of renal injury in group 1: 0. 891P. TNF- 伪 IHS and the pathological score of renal injury in group 1: 0. 891P0. 01NNF- 伪 IHS, and the pathological score of renal injury in group L was positively correlated with that in group 1: r0. 835 (P 0. 01H) and in group 1: 0. 953 (P 0. 01). Conclusion. 1 Acute renal injury model of mice was successfully established by intramuscular injection of 0. 50 mg/kg and 1. 00 mg/kg venom of Agkistrodon halys. 2 the severity of acute renal injury was positively correlated with the dose of Agkistrodon halys venom, and was related to the time of action. 3The expression of TNF- 伪 in renal tissue was positively correlated with the severity of acute renal injury, suggesting that TNF- 伪 might be one of the important inflammatory mediators of acute renal injury induced by Agkistrodon halys venom in mice.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R363

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