大鼠激素性股骨头坏死的2种造模方法比较

发布时间：2018-03-23 23:39

本文选题：股骨头坏死　切入点：激素　出处：《中国药业》2015年20期

【摘要】：目的比较不同地塞米松用药方案腹腔注射建立大鼠激素性股骨头坏死模型的可靠性及成功率,为大鼠激素性股骨头坏死模型的建立方法提供基本数据。方法将30只雄性Wistar大鼠随机分为A组、B组、C组,各10只。A组给予地塞米松磷酸钠注射液20 mg/(kg·d)腹腔注射,1次/周;B组给予地塞米松磷酸钠注射液10 m L/(kg·d)腹腔注射,2次/周;C组给予0.9%氯化钠注射液20 m L/(kg·d)腹腔注射,1次/周;均持续8周。于首次给药8周末摄双髋正位X线检查,次日处死取双侧股骨头标本,行组织形态学检查。结果 A、B、C 3组大鼠股骨头双髋正位X线检查均未发现异常,但在病理切片上可发现显著性差异,证实激素性股骨头坏死造模成功,而B组股骨头坏死改变较A组明显严重。结论腹腔注射地塞米松20 mg/(kg·d),1次/周,持续8周的方案和地塞米松10 m L/(kg·d),2次/周,持续8周的方案均能成功建立大鼠激素性股骨头坏死的早期模型,但后一种造模方案更优。
[Abstract]:Objective to compare the reliability and success rate of steroid-induced femoral head necrosis in rats by intraperitoneal injection of dexamethasone (Dexamethasone). Methods Thirty male Wistar rats were randomly divided into two groups: group A (group B) and group C (group C). 10 rats in group A were given dexamethasone sodium phosphate injection for 20 mg/(kg d (group B was given dexamethasone sodium phosphate injection 10 m L/(kg d) and group C was given 0.9% sodium chloride injection (20 m L/(kg d) once a week. All of them lasted for 8 weeks. At the end of the first 8 weeks of administration, the dual hip positive position X-ray examination was taken, and the specimens of bilateral femoral heads were taken out the next day for histomorphology examination. Results No abnormality was found in the positive position of the femoral heads of the rats in group C, and the results showed that there was no abnormality in the positive position of the femoral heads. However, significant difference was found in pathological sections, which confirmed that steroid-induced femoral head necrosis was successful, while the change of femoral head necrosis in group B was more serious than that in group A. conclusion Dexamethasone is injected intraperitoneally once a week for 20 mg/(kg. The early model of steroid-induced femoral head necrosis in rats was successfully established by the 8-week regimen, dexamethasone 10m L/(kg / week regimen and dexamethasone 10m L/(kg / week regimen, but the latter one was better.
【作者单位】：中国人民解放军医学院;中国人民解放军海军总医院高压氧医学中心;
【基金】：北京市自然科学基金资助项目,项目编号:7154241 全军医学科技青年培育项目,项目编号:13qnp075
【分类号】：R681.8;R-332

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