不同训练方式建立大鼠空间记忆后海马结构中BDNF表达的变化

发布时间：2018-03-26 15:45

本文选题：脑源性神经营养因子　切入点：海马　出处：《华中科技大学学报(医学版)》2015年01期

【摘要】：目的研究短期强化训练能否建立可靠的空间长时记忆;用不同训练方式建立空间记忆后,大鼠海马结构中脑源性神经营养因子(BDNF)的表达是否会发生变化。方法利用Morris水迷宫建立大鼠空间短期记忆、长期记忆和短期强化训练形成的长期记忆3种模型,用激光共聚焦显微镜观察正常(NC)组、短期记忆(ST)组、长期记忆组(LT)和短期强化训练形成的长期记忆(SRT)组大鼠海马各亚区的BDNF的分布情况。结果 Morris水迷宫定位航行实验中LT组和SRT组间寻找站台平均潜伏期和策略差异均无统计学意义;记忆检测发现,LT组大鼠除了在站台所在象限的停留时间明显长于SRT组,差异有统计学意义(P0.05)外,两组大鼠寻找站台潜伏期和策略以及穿越站台次数差异均无统计学意义。LT组与NC组及ST组比较,海马齿状回和CA1区的BDNF免疫荧光反应明显增强,差异有统计学意义(均P0.05);SRT组与NC组以及ST组比较,海马齿状回和CA1区的BDNF免疫荧光反应明显增强,差异有统计学意义(P0.05或P0.01)。LT组与SRT组之间,NC组与ST组之间,海马各区的BDNF表达差异均无统计学意义。结论短期强化训练可建立与长期训练基本相同的长期记忆。BDNF与空间长期记忆的形成密切相关,其可能不参与空间短期记忆的形成或调节。
[Abstract]:Objective to study whether short-term intensive training can establish reliable spatial long-term memory, and to establish spatial memory with different training methods. Whether the expression of brain-derived neurotrophic factor (BDNF) might change in the hippocampal structure of rats. Methods three models of spatial short-term memory, long-term memory and short-term intensive training were established by using Morris water maze in rats. The laser confocal microscope was used to observe the normal group and ST-group. The distribution of BDNF in the hippocampal subareas of rats in the long term memory group (LTT) and the short term intensive training group (LT). Results in the Morris water maze navigation experiment, the average latency and strategy of platform searching between LT group and SRT group were poor. There was no significant difference between the two groups. Memory test showed that the retention time in the quadrant of the LT group was significantly longer than that in the SRT group, and the difference was statistically significant (P 0.05). There was no significant difference in latency, strategy and frequency of platform searching between the two groups. Compared with NC group and St group, the BDNF immunofluorescence reaction in dentate gyrus and CA1 region of Haima dentate gyrus was significantly increased in LT group. The BDNF immunofluorescence reaction in dentate gyrus and CA1 region of Haima japonicus was significantly increased compared with NC group and St group. The difference was statistically significant (P0.05) or between P0.01).LT group and SRT group (P 0.05) and between SRT group and NC group and St group. Conclusion Short-term intensive training can establish the same long-term memory as long-term training, which is closely related to the formation of spatial long-term memory. It may not participate in the formation or regulation of spatial short-term memory.
【作者单位】：西安医学院生理学教研室;西安交通大学医学院生理学与病理生理学系;西安交通大学环境与疾病相关基因教育部重点实验室;
【基金】：教育部中国博士后科学基金资助项目(No.2013 M540760)
【分类号】：R338

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