雷公藤红素降低糖尿病小鼠血糖的机制研究

发布时间：2018-03-27 14:15

本文选题：雷公藤红素　切入点：β-胰岛细胞　出处：《湖北工业大学》2013年硕士论文

【摘要】：目的：雷公藤红素（Celastrol）是一种三萜类化合物，最早从南蛇藤茎中分离得到，具有抗炎，抗癌等多种活性，临床上主要用于治疗类风湿关节炎，皮肤病和肾脏疾病。我们的前期研究表明在适当的剂量下（500μg/kg），雷公藤红素具有很明显的降低由链脉佐菌素（STZ，50mg/kg）所诱导产生糖尿病小鼠血糖的作用。为了进一步探讨雷公藤红素的作用机理，本文通过建立细胞和动物模型，研究了雷公藤红素对其功能的影响。方法：通过炎症因子（IL-1，IL-6）将β-胰岛细胞（INS-1）感染成炎症状态后，加入雷公藤红素（100nM-300nM）治疗处理,观察细胞状态（Immunofluorescence technique）的变化，并测定其功能(KSIS)，细胞凋亡水平（FCM）。建立C57BL/6J小鼠的糖尿病模型，对其体重（BW），随机血糖(GLU)，24小时尿糖(UGE)，尿蛋白（UP），肾功能（Renal Function）等指标进行测定，并提取肾小管上皮细胞总RNA，检测钠葡萄糖共转移蛋白（SGLT）表达的变化。结果：INS-1细胞通过雷公藤红素处理24小时之后，与对照组相比，β-胰岛细胞状态和功能明显好转，细胞凋亡得到了抑制。糖尿病小鼠经雷公藤红素治疗后，血糖明显降低，24小时尿糖增多，尿蛋白增加，，肾小球横截面积增加，SGLT的表达明显降低。结论：雷公藤红素对炎症状态下β-胰岛细胞具有一定的保护作用,通过对钠葡萄糖共转运体的抑制，减少了葡萄糖的重吸收，从而降低糖尿病小鼠的血糖。
[Abstract]:Objective: Tripterygium wilfordii Celastrola is a triterpenoid compound, which was first isolated from the stem of Sagitaria sinensis. It has many anti-inflammatory and anticancer activities, and is mainly used in the treatment of rheumatoid arthritis. Skin diseases and kidney diseases. Our previous studies have shown that tripterygium at an appropriate dose of 500 渭 g / kg, tripteryline can significantly reduce the effect of streptozotocin (STZ) 50 mg / kg on the production of blood glucose in diabetic mice. The mechanism of the action of tripsin, The effects of tripterine on the function of tripterygium wilfordii were studied by establishing cell and animal models. Methods: 尾 -islet cells (INS-1) were infected into inflammatory state by inflammatory cytokine IL-1- (IL-6), then treated with Tripterygium wilfordii 100nM-300nM), the changes of immunofluorescence technique were observed, and the function of KSISI and the level of apoptosis were measured. The diabetic model of C57BL/6J mice was established. The body weight of BWN, 24 hours urine glucose, urine protein, Renal function were measured, and the total RNAs of renal tubular epithelial cells were extracted to detect the expression of sodium and glucose co-transfer protein (SGLTs). Results after treated with tripterine for 24 hours, 尾 -islet cells were significantly improved in state and function, and apoptosis was inhibited after treatment with tripterine in diabetic mice. The expression of SGLT was significantly decreased after 24 hours of blood glucose decreased, urine protein increased and glomerular cross-sectional area increased. Conclusion: Tripterygium wilfordii has a protective effect on 尾 -islet cells in inflammatory state. By inhibiting sodium and glucose co-transporter, tripterygium can reduce the reabsorption of glucose and decrease the blood glucose in diabetic mice.
【学位授予单位】：湖北工业大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R-332;R587.1;R965

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