成年山羊附睾不同区段精子与凝集素芯片结合的差异研究

发布时间：2018-03-28 07:15

本文选题：山羊　切入点：附睾　出处：《中国男科学杂志》2015年05期

【摘要】：目的研究成年山羊精子在附睾成熟过程中与凝集素芯片结合的差异。方法精子分别取自成年山羊附睾头、体、尾部,经2%多聚甲醛/0.2%戊二醛固定后,然后进行碘化丙啶染色标记,再分别与含有91种凝集素的芯片结合。信号经提取后,利用SPSS16.0和R语言对其进行后续分析和比较。结果山羊附睾头、体、尾各部分精子与含各类型糖基特异性的凝集素芯片结合分析后发现,与山羊附睾头部精子结合的凝集素有40种,体部精子结合的有48种,尾部精子结合的有42种。来自附睾不同区段的精子与凝集素结合强度也有显著差异,其中,甘露糖特异性的凝集素与体部精子结合最高,复杂结构特异性的凝集素与附睾头、体、尾各部分精子的结合强度呈梯度下降趋势。结论山羊精子膜表面的糖被,随着精子在附睾中的成熟,也在不断地发生变化的。
[Abstract]:Objective to study the difference of agglutinin microarray binding of adult goat sperm during epididymal maturation. Methods the sperm were taken from the head, body and tail of adult goat epididymis and fixed with 2% paraformaldehyde / 0.2% glutaraldehyde. After extraction, the signals were analyzed and compared by SPSS16.0 and R language. Results the goat epididymal head, body, body, head and body of epididymis were analyzed and compared with each other by staining with pyridine iodide and binding to the chip containing 91 kinds of lectins, respectively. After binding to lectin microarray containing various types of glycosylated sperm, 40 kinds of agglutinin binding to goat epididymal head sperm and 48 kinds of body sperm binding were found. The binding intensity of sperm from different regions of epididymis to agglutinin was also significantly different. Among them, mannose specific lectin had the highest binding to body sperm, and complex structure specific lectin was associated with epididymal head. Conclusion the sugars on the surface of sperm membrane on goat spermatozoa are changing with the maturation of sperm in epididymis.
【作者单位】：华东理工大学生物反应器工程国家重点实验室;上海交通大学系统生物医学研究院;国家人口和计划生育委员会计划生育药具重点实验室上海市计划生育科学研究所;中国科学院上海生命科学研究院生物化学与细胞生物学研究所;
【基金】：国家青年科学基金项目(81401252)
【分类号】：R339.2

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