大鼠心理应激模型建立及相关因素评价

发布时间：2018-03-29 16:33

本文选题：心理应激　切入点：HPA轴　出处：《吉林大学》2010年硕士论文

【摘要】： 本研究是在下丘脑-垂体-肾L腺皮质轴理论基础上,建立大鼠心理应激模型,将大鼠随机分为正常对照组、急性应激组、慢性应激组,以大鼠血清ACTH含量测定评价如何建立稳定的动物模型,以大鼠应激后心电图及心肌病理组织学观察评价应激所致心肌损伤。结果显示：(1)慢性应激组血清ACTH含量较正常对照组比较明显升高,差异具有统计学意义(P0.01)。(2)刺激2周后,脱离应激原1天,在心电图记录中,大鼠心律失常及各波段变化明显多于应激前和对照组,表现为窦性心动过速、房性、室性期前收缩、RR间期明显缩短、ST段抬高或下移、T波高耸直立或低平、病理性Q波。(3)心肌组织免疫组化染色结果显示：急、慢性应激后心肌组织热休克蛋白70表达均有不同程度增多。(4)心肌细胞电镜下超微结构显示：线粒体肿胀、变圆,出现空泡样变等改变,心肌细胞核周间隙扩张,染色质浓集、边缘化。肌膜增厚模糊、肌节扭曲、肌丝排列紊乱。随着应激时间延长,发现肌丝断裂、溶解,甚至消失,线粒体排列紊乱,呈凝集状、外膜模糊、内嵴不清,基底致密等退行性改变。由以上结果可以得出以下结论：急、慢性应激方法建立的大鼠心理应激模型,其HPA轴反应性均增高,存在着确切的心律失常和明显的心肌组织损伤,是一个稳定的应激模型,有利于进一步研究其miRNA变化。
[Abstract]:On the basis of hypothalamus-pituitary-kidney L-gland axis theory, a rat model of psychological stress was established. The rats were randomly divided into normal control group, acute stress group and chronic stress group. To evaluate how to establish a stable animal model by measuring the content of serum ACTH in rats. The myocardial injury induced by stress was evaluated by electrocardiogram (ECG) and cardiomyopathology histological observation of rats after stress. The results showed that the serum ACTH content in the chronic stress group was significantly higher than that in the normal control group, and the difference was statistically significant after 2 weeks of stimulation. In the electrocardiogram recording of isolated stressors, arrhythmia and the changes of each band were significantly more than those before stress and control group, showing sinus tachycardia, atrial tachycardia, atrial tachycardia, atrial tachycardia, atrial tachycardia. Ventricular precontractile RR interval significantly shortened St segment elevation or downshift T wave tall or low level, pathological Q wave. 3) the results of immunohistochemical staining showed that: acute; After chronic stress, the expression of heat shock protein 70 (HSP70) in myocardial tissue increased in varying degrees. The ultrastructure of myocardial cells under electron microscope showed that mitochondria were swollen, rounded, vacuolated, myocardial perinuclear space dilated, chromatin concentrated, etc. Marginalization. Myomembranous thickening and blurring, sarcomere twisting, myofilament arrangement disorder. With the prolongation of stress time, it is found that myofilament breaks, dissolves, and even disappears, mitochondria are arranged disorderly, showing agglutination, outer membrane blur, inner cristae unclear, From the above results, the following conclusions can be drawn: the HPA axis reactivity of the psychological stress model established by acute and chronic stress methods in rats is all increased, and there are definite arrhythmias and obvious myocardial tissue damage. It is a stable stress model, which is helpful to further study the change of miRNA.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R-332

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