构建新西兰白兔脊柱结核模型的实验研究

发布时间：2018-03-30 04:18

本文选题：动物模型　切入点：脊柱结核　出处：《新疆医科大学》2010年硕士论文

【摘要】：目的:在新西兰白兔腰椎局部钻孔种植人型结核杆菌H37Rv标准菌株构建脊柱结核疾病模型,为以后骨结核的基础研究提供动物模型。方法:对未致敏的新西兰白兔的腰5椎体上方近椎间盘处钻骨孔,填充明胶海绵,吸附结核菌悬液(菌量:0.5mg/0.1ml)建立模型,同时设置生理盐水对照组及空白组,术后行大体观察、影像学、组织病理学、细菌学等检查证实。结果:新西兰白兔腰椎在行H37Rv结核菌株感染后,局部反应较明显,全身反应较轻。感染组9只兔完成实验(9/12),其中3只出现消瘦、纳差等症状(3/9);术后1月X线阳性兔2只(2/9),术后2月X线阳性兔4只(4/9),术后2月CT阳性兔6只(6/9),术后2月MRI阳性兔7只(7/9);感染组阳性兔的椎体、椎旁软组织切片HE染色示有脓细胞、类上皮样细胞或坏死灶等形成,感染组中脓肿形成的兔脓液培养示60.0%结核分枝杆菌生长。建立模型成功率为66.7%(6/9)。结论:通过在未致敏的兔脊柱椎体局部进行适当剂量的H37Rv结核标准菌株的种植感染,可以构建出与人类脊柱结核病理变化相似的兔脊柱结核。通过症状、影像、组织病理、细菌学等手段,可以成功地证实模型。
[Abstract]:Objective: to establish a model of spinal tuberculosis disease in New Zealand white rabbit lumbar vertebrae implanted with standard strain of Mycobacterium tuberculosis (H37Rv). Methods: an animal model was established on the unsensitized New Zealand white rabbits with bone holes near the intervertebral disc above the lumbar 5 vertebrae, filling with gelfoam, and adsorbing the suspension of tuberculous bacteria (0. 5 mg / 0.1 ml). At the same time, normal saline control group and blank group were set up, the gross observation, imaging, histopathology and bacteriology were performed after operation. Results: the local reaction of the lumbar vertebrae of New Zealand white rabbits was obvious after the infection of H37Rv tuberculous bacilli. The whole body reaction was mild. Nine rabbits in the infected group completed the experiment 9 / 12, and 3 of them were emaciated. Anorexia and other symptoms were 3 / 9 / 9; X-ray positive rabbits were 2 / 9 / 9 in 1 month after operation, 4 / 9 / 9 in X ray positive rabbits in 2 months after operation, 6 / 9 / 9 in CT positive rabbits in 2 months after operation, 7 / 9 / 9 in MRI positive rabbits in 2 months after operation; vertebrae of positive rabbits in infection group. He staining of paravertebral soft tissue sections showed the formation of pyogenic cells, epithelioid cells or necrotic foci. In the infection group, the rabbit abscess culture showed that the mycobacterium tuberculosis grew in 60.0%. The success rate of the model was 66.70.Conclusion: the appropriate dose of H37Rv TB standard strain was implanted in the unsensitized spinal vertebrae of rabbits. The rabbit spinal tuberculosis which is similar to the pathological changes of human spinal tuberculosis can be successfully confirmed by means of symptoms, images, histopathology, bacteriology and so on.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R-332;R529.2

【引证文献】