宠物犬蓝氏贾第鞭毛虫和隐孢子虫感染的分子检测

发布时间：2018-04-01 19:09

  本文选题：蓝氏贾第鞭毛虫　切入点：隐孢子虫　出处：《中国寄生虫学与寄生虫病杂志》2015年05期

【摘要】：目的了解皖浙两省部分城镇地区宠物犬蓝氏贾第鞭毛虫和隐孢子虫感染情况,及聚集体类型或虫种。方法在安徽多地和浙江杭州共采集315份新鲜宠物犬粪样,分别采用基于蓝氏贾第鞭毛虫谷氨酸脱氢酶(GDH)基因的半巢式PCR和隐孢子虫核糖体小亚基(SSU r RNA)基因的巢式PCR方法对所有粪样进行扩增,并对获得的阳性扩增产物进行测序和生物信息学分析,确定蓝氏贾第鞭毛虫的聚集体和隐孢子虫的虫种类型。结果 315份犬粪样中,蓝氏贾第鞭毛虫和隐孢子虫的阳性率分别为3.2%(10/315)和1.6%(5/315)。幼龄犬(≤12个月)蓝氏贾第鞭毛虫阳性率(17.8%)和隐孢子虫阳性率(11.1%)均显著高于成年犬(0.7%和0)(P0.05)。雌性犬和雄性犬蓝氏贾第鞭毛虫和隐孢子虫阳性率差异均无统计学意义(P0.05)。发现蓝氏贾第鞭毛虫两种聚集体类型,即聚集体B(n=6)和聚集体D(n=4)。从5份阳性犬粪中分离的隐孢子虫经巢式PCR SSU r RNA基因检测,均为犬隐孢子虫(Cryptosporidium canis)。结论皖浙两省部分地区宠物犬蓝氏贾第鞭毛虫和犬隐孢子虫的阳性率分别为3.2%和1.6%。发现的蓝氏贾第鞭毛虫聚集体类型为聚集体B和聚集体D。
[Abstract]:Objective to investigate the infection of Giardia lamblia and Cryptosporidium in pet dogs in some cities and towns of Anhui and Zhejiang provinces. Methods 315 fresh dog feces were collected in Anhui Province and Hangzhou Zhejiang Province. All fecal samples were amplified by nested PCR and Cryptosporidium ribosomal small subunit SSU r RNAs, respectively, based on the GDH gene of Giardia lamblia. The positive amplified products were sequenced and bioinformatics analysis to determine the aggregates of Giardia lamblia and the species types of Cryptosporidium. The positive rates of Giardia lamblia and Cryptosporidium were 3.210 / 315) and 1.6 / 315g respectively. The positive rates of Giardia lamblia and Cryptosporidium in young dogs (鈮，

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