河南省福氏志贺菌株中毒力相关基因及She毒力岛的分布研究

发布时间：2018-04-02 17:17

本文选题：福氏志贺菌杆菌　切入点：She岛　出处：《大连医科大学》2008年硕士论文

【摘要】： 痢疾杆菌是一种危害人类健康的重要病原体,每年全球有1.6亿人感染痢疾杆菌,引起1100万死亡,其中大多为5岁以下的儿童和老人。在我国每年1000万病例中,大多是由福氏志贺菌株引起的。另外,痢疾杆菌致病与菌株的毒力基因密切相关,因此,了解菌株中毒力相关基因及毒力岛的分布情况对痢疾的防控具有重要意义。在本研究中,我们采用PCR和打点杂交的方法检测了sat、ial、sen、setA、ipaH、icsP、sigA、pic、iutA、icsA 10个毒力相关基因在河南省2002、2003、2005年三年间分离的222株福氏志贺菌株中的分布情况。同时,对这些菌株中She毒力岛的分布情况进行了初步研究。结果发现,所检测的S.flexneri所有菌株中, sat、sen、ipaH、icsP、iutA、icsA基因的阳性率为100%(222 /222);而ial基因的阳性率为99.1%(220/222)。在所有S.flexneri菌株中,有12株菌检测不到setA、sigA、pic基因;有6株菌缺失了She毒力岛。本研究为我们提供了河南省3年不同血清型福氏志贺菌中毒力相关基因的分布情况,将对我国痢疾杆菌的防治与控制具有指导意义。
[Abstract]:Shigella dysenteriae is an important pathogen harmful to human health. Every year, 160 million people worldwide are infected with Shigella, causing 11 million deaths, most of which are children and old people under 5 years of age.Most of the 10 million cases in China are caused by Shigella flexneri.In addition, the virulence genes are closely related to the pathogenicity of Shigella. Therefore, it is important to understand the distribution of virulence genes and virulence islands in the prevention and control of dysentery.In this study, PCR and dot hybridization were used to detect the distribution of 10 virulence related genes in 222 Shigella flexneri strains isolated from Henan Province from 2002 to 2005.At the same time, the distribution of She virulence island in these strains was studied.The results showed that the positive rate of S.flexneri was 100% 222 / 222%, while the positive rate of ial gene was 99.1 / 220%.This study provides us with the distribution of genes related to the toxicity of Shigella flexneri for 3 years in Henan Province, which will be of guiding significance for the prevention and control of Shigella flexneri in China.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R378

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