miR-708-5p通过抑制TMEM88表达促进人骨髓间充质干细胞迁移

发布时间：2018-04-03 17:01

  本文选题：人骨髓间充质干细胞　切入点：MicroRNA--p　出处：《中国病理生理杂志》2015年02期

【摘要】：目的:研究年龄相关microRNA-708-5p(miR-708-5p)对人骨髓间充质干细胞(h MSCs)迁移能力的调控作用。方法:通过microRNA芯片和real-time PCR检测供体年龄对h MSCs中miR-708-5p表达的影响;通过转染miR-708-5p模拟物或抑制物,过表达或抑制miR-708-5p表达;通过细胞划痕及Transwell实验检测h MSCs迁移能力。通过siRNA研究miR-708-5p靶基因跨膜蛋白88(TMEM88)对h MSCs中β链球蛋白(β-catenin)及h MSCs迁移功能的影响。结果:随供体年龄增加,h MSCs中miR-708-5p的表达下降。过表达miR-708-5p可促进h MSCs迁移。相反,抑制miR-708-5p的表达可减少h MSCs迁移。随供体年龄增加,TMEM88表达增加,而β-catenin表达下降,直接抑制TMEM88的表达可促进β-catenin表达,促进h MSCs迁移。同时抑制miR-708-5p和TMEM88,使miR-708-5p失去对h MSCs的调控作用。结论:miR-708-5p可通过抑制TMEM88表达,上调β-catenin,从而活化Wnt/β-catenin信号通路,促进h MSCs迁移。
[Abstract]:Aim: to investigate the effect of age-related microRNA-708-5pmmiR-708-5p on the migration of human bone marrow mesenchymal stem cells (MSCs).Methods: the effect of donor age on the expression of miR-708-5p in h MSCs was detected by microRNA microarray and real-time PCR, the expression of miR-708-5p was over-expressed or inhibited by transfection of miR-708-5p mimics or inhibitors, and the migration ability of h MSCs was detected by cell scratch and Transwell experiments.The effects of miR-708-5p target gene transmembrane protein 88 (TMEM88) on 尾 -catenin (尾 -catenin) and the migration function of h MSCs in h MSCs were studied by siRNA.Results: the expression of miR-708-5p decreased with the age of donor.Overexpression of miR-708-5p can promote the migration of h MSCs.On the contrary, inhibiting the expression of miR-708-5p reduced the migration of h MSCs.The expression of 尾 -catenin increased with the increase of donor age, while the expression of 尾 -catenin decreased. Directly inhibiting the expression of TMEM88 could promote the expression of 尾 -catenin and promote the migration of h MSCs.Both miR-708-5p and TMEM88 were inhibited, which caused miR-708-5p to lose its regulatory effect on h MSCs.Conclusion: 1 miR-708-5p can activate Wnt/ 尾 -catenin signaling pathway and promote h MSCs migration by inhibiting the expression of TMEM88 and up-regulating 尾 -catenin.
【作者单位】： 广州医科大学附属第二医院广州心血管疾病研究所;广州医科大学附属第二医院心内科;广州医科大学附属第二医院肿瘤科;
【基金】：国家自然科学基金青年基金资助项目(No.81401156) 广州市医药卫生科技项目(No.20141A011088)
【分类号】：R329.2
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本文编号：1706101