脑室注射脂多糖建立小鼠认知行为障碍模型

发布时间：2018-04-04 18:21

本文选题：脂多糖　切入点：认知功能　出处：《解放军医学院学报》2015年05期

【摘要】：目的探讨脂多糖(lipopolysaccharide,LPS)单次脑室注射对C57小鼠认知功能的影响,以期建立中枢炎症的认知功能障碍小鼠模型。方法将40只C57小鼠分为空白对照组、LPS 10 ng组、LPS 100 ng组与LPS 2 000 ng组,进行Morris水迷宫空间获得性训练后分别对脑室单次给予LPS 0 ng、10 ng、100 ng、2 000 ng,给药后1 d进行空间探索实验,1~3 d进行工作记忆检测;另将40只C57小鼠分为空白对照组、LPS 10 ng组、LPS 100 ng组与LPS 2 000 ng组,进行新物体识别实验,适应并受训后进行脑室给药,给药后1 d行新物体识别测试;取10只C57小鼠分为两组,分别对脑室给予人工脑脊液、LPS 2 000 ng,6 h后取材检测海马IL-1β、TNF-α含量。结果与空白对照组相比,脂多糖给药组均引起小鼠在Morris水迷宫和新物体识别实验中认知功能下降,其中2 000 ng LPS给药组分别引起水迷宫空间探索实验中小鼠穿台次数、平台周边距离、目标象限停留路程百分比均分别减少72%、46%、32%,工作记忆检测中登台潜伏期延长2倍;新物体识别实验中新物体探索时间、探索次数百分比分别降低22%、21%;ELISA结果显示,2 000 ng LPS脑室注射导致海马IL-1β、TNF-α含量分别升高55%、41%。结论单次LPS(2μg)脑室注射可引起C57小鼠认知功能障碍,提示LPS导致炎性损伤可以作为小鼠认知功能障碍的候选模型。
[Abstract]:Objective to investigate the effect of single intraventricular injection of lipopolysaccharide (LPS) on cognitive function in C57 mice, and to establish a model of cognitive dysfunction in mice with central inflammation.Methods 40 C57 mice were divided into two groups: control group (10 ng) and LPS group (2 000 ng).After Morris water maze spatial acquired training, LPS 0 ng10 ng10 ng10 ng1 000 ng / g was given to the ventricle, and 1 day later, the space exploration experiment was carried out for 3 days to detect working memory.In addition, 40 C57 mice were divided into two groups: blank control group, LPS-10ng group, LPS-100ng group and LPS 2000ng group. The new object recognition experiment was carried out after adaptation and training, and the new object recognition test was performed 1 day after administration, and 10 C57 mice were divided into two groups, one day after administration, 10 C57 mice were divided into two groups.The contents of IL-1 尾 -TNF- 伪 in hippocampus were detected by intraventricular administration of LPS-2 000 ng / L for 6 h.Results compared with the blank control group, the lipopolysaccharide group caused the decrease of cognitive function in the Morris water maze and the new object recognition experiment, and the 2 000 ng LPS group caused the mice to wear the platform in the water maze space exploration experiment respectively.The distance around the platform and the percentage of the distance in the quadrant of the target were reduced by 722 / 46/ 3232, respectively, and the incubation period of the platform was increased by 2 times in the working memory test, and the exploration time of the new object in the new object recognition experiment was also increased by 2 times.The results of Elisa showed that the intraventricular injection of 2 000 ng LPS increased the content of IL-1 尾 -TNF- 伪 in hippocampus and increased the content of TNF- 伪 in hippocampus by 55% and 41%, respectively.Conclusion Intraventricular injection of LPS(2 渭 g can induce cognitive dysfunction in C57 mice, suggesting that LPS induced inflammatory injury can be used as a candidate model for cognitive dysfunction in C57 mice.
【作者单位】：解放军总医院麻醉手术中心;北京军事医学科学院毒物药物研究所;
【基金】：国家自然科学基金项目(81371204)~~
【分类号】：R749.1;R-332

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