钛表面喷砂-微弧氧化对骨髓间充质干细胞黏附和增殖的影响

发布时间：2018-04-05 03:27

本文选题：喷砂酸蚀法　切入点：微弧氧化法　出处：《广东医学》2015年12期

【摘要】：目的比较喷砂酸蚀法(SLA)、微弧氧化法(MAO)和喷砂-微弧氧化法(SL-MAO)处理钛植入体表面对骨髓间充质干细胞(BMMSCs)黏附和增殖能力的影响。方法采用SLA、MAO和SL-MAO处理钛表面(分别为SLA组、MAO组、SL-MAO组),通过扫描电子显微镜(SEM)分析表面结构特征。分离培养SD大鼠BMMSCs,采用SEM观察细胞黏附情况,噻唑蓝法定量分析细胞增殖情况。结果 SLA组钛表面形成微米-亚微米复合多孔,但存在喷砂颗粒残留。MAO组钛表面形成亚微米级微孔,但无微米级大孔结构。SL-MAO组不仅能形成微米-亚微米复合多孔形貌,而且喷砂颗粒残留现象减小。BMMSCs细胞黏附和MTT法增殖试验显示:第1~9天期间,SL-MAO组细胞数量与MAO组差异无统计学意义(P0.05),MAO组和SL-MAO组细胞早期黏附和增殖速率均显著高于SLA组(P0.05)。结论 SL-MAO能在钛植入体表面形成良好的微米-亚微米复合多孔形貌,其复合多级孔结构优于单纯使用微弧氧化处理,在膜层的完整性方面优于传统的喷砂酸蚀处理。在BMMSCs细胞早期黏附和促进细胞增殖方面,SL-MAO相比SLA具有显著优势,与MAO差异不显著。
[Abstract]:Objective to compare the effect of SLAA, MAO and SL-MAO on the adhesion and proliferation of bone marrow mesenchymal stem cells (BMMSCs) treated with titanium implantation.Methods\\\BMMSCs were isolated and cultured in SD rats. Cell adhesion was observed by SEM and cell proliferation was quantitatively analyzed by thiazolyl blue method.Results the micrometer / submicron composite porous was formed on the titanium surface of SLA group, but there were submicron micropores on the surface of TIO group with sand blasting particles. However, the micrometer composite porous morphology was not only formed in the SL-MAO group without micrometer macroporous structure.The cell adhesion and proliferation test by MTT method showed that there was no significant difference between SL-MAO group and MAO group in cell adhesion and proliferation rate between P0.05 and SL-MAO groups during the first nine days. The rate of early cell adhesion and proliferation in SL-MAO group was significantly higher than that in SLA group.Conclusion SL-MAO can form a good micromicron submicron composite porous morphology on the surface of titanium implants. The composite multilevel pore structure is superior to that of single micro-arc oxidation treatment, and the integrity of the film layer is superior to that of the traditional sand blasting etching treatment.SL-MAO has significant advantages over SLA in early adhesion and proliferation of BMMSCs cells, but there is no significant difference between SL-MAO and MAO.
【作者单位】：广东省口腔医院;南方医科大学附属口腔医院正畸科;四川大学华西口腔医院正畸科口腔疾病研究国家重点实验室;
【基金】：广东省医学科研基金资助项目(编号:B2014035)
【分类号】：R329.2

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