颅咽管瘤鸡胚绒毛尿囊膜移植瘤模型的建立

发布时间：2018-04-05 09:06

本文选题：颅咽管瘤　切入点：鸡胚绒毛尿囊膜　出处：《中国神经精神疾病杂志》2015年11期

【摘要】：目的初步建立颅咽管瘤鸡胚绒毛尿囊膜(chorioallantoic membrane CAM)移植瘤模型,并评估移植瘤血管生成能力及细胞增殖能力。方法利用术中所得颅咽管瘤组织,异种移植到鸡胚CAM表面,建立移植瘤模型。对移植瘤瘤体周围CAM血管进行测定,评估移植瘤CAM血管生成能力,同时利用免疫组化法以CD34标记移植瘤微血管密度(microvessel density,MVD),以PCNA检测移植瘤细胞增殖活性。结果成功建立颅咽管瘤CAM移植瘤模型,造釉细胞型颅咽管瘤成瘤率为47.14%,鳞状上皮型颅咽管瘤成瘤率为43.33%,两者无显著差异(χ2=0.123,P=0.726);造釉细胞型移植瘤瘤周CAM血管生成能力,微血管密度及细胞增殖活性均高于鳞状上皮型移植瘤。移植瘤PCNA的表达与MVD(Pearson r=0.639,P0.001)及CAM血管评分(Spearman r=0.490,P=0.001)呈正相关。结论采用异位异种移植的方法可在鸡胚CAM上建立颅咽管瘤移植瘤模型。移植瘤可不同程度地刺激CAM上血管生成,移植瘤自身可建立微循环,具有一定的增殖能力。
[Abstract]:Objective to establish the model of chorioallantoic membrane (CAM) transplanted into the chorioallantoic of craniopharyngioma, and to evaluate the angiogenesis and cell proliferation of the transplanted tumor.Methods using craniopharyngioma tissues obtained during operation, xenografts were transplanted to the CAM surface of chicken embryo, and the transplanted tumor model was established.The CAM vessels around the transplanted tumor were measured to evaluate the ability of CAM angiogenesis, and the microvessel density MVD was labeled with CD34 by immunohistochemical method, and the proliferative activity of transplanted tumor cells was detected by PCNA.The microvessel density and cell proliferation activity were higher than those of squamous epithelial transplanted tumor.The expression of PCNA in transplanted tumors was positively correlated with the expression of MVD(Pearson rnr 0.639m P0.001 and CAM vascular score of 0.490 P0. 001).Conclusion the transplantation model of craniopharyngioma on chicken embryo CAM can be established by heterotopic xenotransplantation.The transplanted tumor can stimulate angiogenesis on CAM to some extent, and the transplanted tumor itself can establish microcirculation and has certain proliferative ability.
【作者单位】：福建医科大学附属第一医院神经外科;南方医院神经外科;四川医科大学附属第一医院神经外科;
【基金】：福建省卫计委青年科研项目(编号:) 福建省自然科学基金(编号:2015J05150)
【分类号】：R739.41;R-332

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