静磁场影响机体微循环系统的初步机理研究

发布时间：2018-04-10 09:05

本文选题：静磁场　切入点：微循环　出处：《第四军医大学》2010年硕士论文

【摘要】： 磁场是否能治疗疾病?这个古老而又悬而未决的话题一直困扰科学界多年,依然没有定论;同时,随着磁疗技术和仪器在临床上的广泛应用,回答磁场是否会对人体产生不良影响这个问题就显得非常紧迫。静磁场对机体微循环系统的影响研究作为磁场生物效应的一个重要分支,日益受到广泛重视。根据对国内外文献的回顾,本文综述了该领域复杂的研究现状,分析了该领域经常出现复杂矛盾和尚不明朗的实验结果的潜在原因。通过建立磁场的计算机仿真模型,进一步进行磁场对动物和人体微循环的影响实验,采用激光多普勒技术和连续小波变换技术评估了磁场暴露后局部微循环的指标的改变情况,初步探讨了磁场对机体微循环系统影响的机理,以期对该领域的进一步研究提供新的方向。主要工作概括如下: 一、首次利用Comsol Multiphysics 3.4软件系统模拟了钕铁硼型磁片及磁片组、螺线管型交变电磁场和脉冲电磁场的三维时空分布,很好的弥补了传统磁场生物效应实验中对电磁场的理论分析不够直观和形象的缺点。通过统计和数值分析方法对仿真结果与实际测量数据进行对比分析,证实模拟结果精确度高,可以作为对实测结果和理论分析的很好的补充。在计算精度要求不是很高的情况下,甚至可以代替理论分析进行计算,为一些不规则复杂磁场的分布问题提供了很好的解决方案。二、采用上表面中心强度约为220mT的静磁场(较小尺寸)对正常小鼠耳廓微循环进行30min短时暴露,发现在暴露中期和暴露后期,该尺寸和强度的静磁场未对小鼠耳廓微循环的血流量产生明显影响。关于该强度和该部位的研究国内外未见报道。三、采用了基于连续小波频谱分析的新技术来评估上表面中心强度约为220mT的静磁场(较大尺寸)30min短期暴露对正常青年甲襞微循环系统的影响。结果发现,在暴露中期,磁场可能会引起血管平滑肌肌源性活动、外周神经系统的活动和内皮相关的代谢活动的增强(P0.05),而整体的血流量的均值没有显著性变化(P0.05),仍然保持在基值附近。此外,撤掉磁场之后,静磁场暴露中期出现的血流的节律变化逐渐消失,说明该强度静磁场对人体微循环系统影响的延迟作用并不明显。首次结合电磁屏蔽技术和小波频谱分析对局部微血流的无创实时监控技术研究磁场对人体微循环的影响,同时首次通过活体实验验证了磁场可能会潜在地影响正常人体的微循环调控通路,为该领域的进一步深入研究提供新的评估手段和思路。
[Abstract]:Can magnetic fields cure diseases?This ancient and unresolved topic has been troubling the scientific community for many years and remains inconclusive; at the same time, with the widespread use of magnetic therapy techniques and instruments in the clinic,It is urgent to answer the question of whether magnetic fields have adverse effects on the human body.As an important branch of the biological effect of magnetic field, the effect of static magnetic field on microcirculation system has been paid more and more attention.Based on the review of domestic and foreign literatures, this paper summarizes the current situation of complex research in this field, and analyzes the potential causes of complex contradictions and unclear experimental results in this field.By establishing the computer simulation model of magnetic field, the effect of magnetic field on animal and human microcirculation is further tested. The changes of local microcirculation index after magnetic field exposure are evaluated by laser Doppler technique and continuous wavelet transform technique.The mechanism of the effect of magnetic field on microcirculation system is discussed in order to provide a new direction for further research in this field.The main tasks are summarized as follows:For the first time, Comsol Multiphysics 3.4 software is used to simulate the three-dimensional spatial and temporal distribution of the NdFeB magnets, magnetic disk sets, solenoid alternating electromagnetic fields and pulsed electromagnetic fields for the first time.It makes up for the shortcoming of the theory analysis of electromagnetic field in the traditional experiment of biological effect of magnetic field.The simulation results are compared with the actual measured data by means of statistical and numerical analysis. It is proved that the simulation results are accurate and can be used as a good supplement to the measured results and the theoretical analysis.When the precision is not very high, it can even replace the theoretical analysis and provide a good solution for the distribution of some irregular and complex magnetic fields.Second, 30min exposure to the auricle microcirculation of normal mice was carried out by using a static magnetic field (smaller size) with a central intensity of 220mT on the upper surface. It was found that in the middle and late stages of exposure, the microcirculation of the auricle of normal mice was exposed for a short time.The size and intensity of the static magnetic field did not significantly affect the blood flow of auricle microcirculation in mice.There are no reports about the strength and the site.Thirdly, a new technique based on continuous wavelet spectrum analysis was used to evaluate the effect of the magnetostatic magnetic field (220mT) on the nailfold microcirculation system of the normal young people.It was found that in the middle of exposure, the magnetic field may induce the myogenic activity of vascular smooth muscle.The activity of peripheral nervous system and the enhancement of endothelium-related metabolic activity (P0.05A), while the mean of whole blood flow was not significantly changed (P0.05), and remained near the base value.In addition, after removing the magnetic field, the rhythm of the blood flow in the middle period of the exposure gradually disappeared, indicating that the delayed effect of the intensity of the static magnetic field on the human microcirculation system is not obvious.It is the first time to study the effect of magnetic field on human microcirculation by combining electromagnetic shielding technology and wavelet spectrum analysis to non-invasive real-time monitoring of local micro blood flow.At the same time, the experiment in vivo for the first time proves that the magnetic field may potentially affect the regulation pathway of microcirculation in normal human body, which provides a new evaluation method and thought for further research in this field.
【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R312

【参考文献】