大鼠煤工尘肺动物模型的研究

发布时间：2018-04-10 17:25

本文选题：大鼠 + 煤工尘肺　；参考：《吉林大学》2009年博士论文

【摘要】： 本研究目的旨在建立一种更为合适的安全性高、重复性好、准确、经济、易于制作和评价的煤工尘肺大鼠动物模型。采用气溶胶吸入法和非暴露气管灌注法2种染尘方式建立大鼠煤工尘肺动物模型,分别于染尘后2周、4周、8周、3个月、6个月、9个月、12个月、18个月检测各组实验动物肺功能、血气分析、支气管肺泡灌洗液细胞成分、肺泡灌洗液中TNF-α及TGF-β水平、支气管肺泡灌洗液细胞TNF-α及TGF-β的mRNA表达水平、肺泡巨噬细胞非特异性吞噬功能、肺组织病理、肺组织内羟脯氨酸的含量等指标;并比较各组所制动物模型不同时间、各项指标变化趋势及相关性。通过此项研究,我们首先建立了煤尘气溶胶吸入装置及煤尘气溶胶的制备,并成功采用非暴露气管灌注法和气溶胶吸入法2种染尘方式建立大了鼠煤工尘肺动物模型,两种方法均准确、经济地复制了大鼠煤工尘肺动物模型,以高浓度暴露更为理想;但非气管暴露灌注法对试验者实验技术复杂、动物死亡率较高、短时间内可复制动物模型;气溶胶吸入法动物死亡率低、符合煤工尘肺致病因素和发病环境及煤工尘肺的发生发展过程,简便、易于制作和评价,更为适用于煤工尘肺发病机制、诊断及治疗的临床研究。
[Abstract]:The purpose of this study was to establish a more suitable animal model of coal worker pneumoconiosis with high safety, good reproducibility, accuracy, economy and easy to be made and evaluated.The animal models of coal worker pneumoconiosis were established by aerosol inhalation and non-exposed tracheal instillation. The lung function of experimental animals in each group was measured at 4 weeks, 8 weeks, 3 months, 6 months, 9 months, 12 months and 18 months after dust exposure, respectively.Blood gas analysis, cell composition of bronchoalveolar lavage fluid, TNF- 伪 and TGF- 尾 levels in bronchoalveolar lavage fluid, mRNA expression level of TNF- 伪 and TGF- 尾 in bronchoalveolar lavage fluid cells, nonspecific phagocytosis of alveolar macrophages, lung histopathology.The content of hydroxyproline in lung tissue was compared and the change trend and correlation of each index were compared.Through this study, we first established the coal dust aerosol inhalation device and the preparation of coal dust aerosol, and successfully established the animal model of coal worker pneumoconiosis by two kinds of dusting methods: unexposed trachea perfusion method and aerosol inhalation method.The two methods were both accurate and economically duplicated the animal model of coal worker pneumoconiosis in rats, but the non-tracheal exposure perfusion method was more suitable for the experimental technique of the experimenter, the mortality rate of the animal was higher, and the animal model could be reproduced in a short time.The aerosol inhalation method is suitable for the clinical study of pathogenesis, diagnosis and treatment of coal pneumoconiosis, because of its low mortality rate, consistent with the pathogenic factors and environment of coal pneumoconiosis and the process of occurrence and development of coal worker pneumoconiosis. It is simple and easy to make and evaluate.
【学位授予单位】：吉林大学
【学位级别】：博士
【学位授予年份】：2009
【分类号】：R135.2;R-332

【引证文献】