通络汤对腰椎间盘突出症模型大鼠的影响

发布时间：2018-04-11 04:28

本文选题：腰椎间盘突出症 + 通络汤　；参考：《中国现代应用药学》2015年07期

【摘要】：目的探讨通络汤对腰椎间盘突出症模型大鼠的治疗作用。方法 48只大鼠随机分为6组:正常组、模型组、阳性对照组、通络汤高、中、低剂量组,除正常组外,各组均通过手术建立大鼠自体尾髓核移植所致机械性压迫髓核突出动物模型。热板法测定痛阈,玻片法测定凝血时间,血液流变仪测定血液流变学指标,酶免法检测肿瘤坏死因子-α(TNF-α)、前列腺素E2(PGE2)、一氧化氮(NO)含量和一氧化氮合成酶(NOS)活性;取神经根进行病理学检测。结果大鼠腰突症模型建立后痛阈降低,凝血时间缩短,血液黏度增大,血清NO、NOS、TNF-α、PGE2含量升高,神经根组织出现炎性浸润。通络汤可显著改善这些症状,表现为提高痛阈,延长凝血时间,降低血液黏度,抑制血清中炎症因子NO、NOS、TNF-α、PGE2的产生,减少神经根组织炎性浸润的产生。结论通络汤能够有效缓解腰椎间盘突出症疼痛症状,这可能与减轻神经根组织的充血、水肿、炎症反应,降低全血黏度、血浆黏度,延长凝血时间,改善血液循环等有关。
[Abstract]:Objective to investigate the therapeutic effect of Tongluo decoction on lumbar disc herniation model rats.Methods 48 rats were randomly divided into 6 groups: normal group, model group, positive control group, Tongluo decoction high, middle and low dosage group, except normal group.The rat models of nucleus pulposus protruding mechanically compressed by autologous tail nucleus transplantation were established by operation in each group.Pain threshold was measured by hot plate method, coagulation time was measured by glass plate method, hemorheology index was measured by hemorheometer, tumor necrosis factor- 伪 -TNF- 伪, prostaglandin E2PGE2N, nitric oxide nitrite (no) and nitric oxide synthase (NOS) activity were measured by enzyme immunoassay (Elisa).Nerve roots were taken for pathological examination.Results the pain threshold decreased, coagulation time shortened, blood viscosity increased, serum NONSO TNF- 伪 PGE2 content increased and inflammatory infiltration occurred in nerve root tissue after the establishment of lumbar process model in rats.Tongluo decoction can significantly improve these symptoms, such as increasing pain threshold, prolonging coagulation time, reducing blood viscosity, inhibiting the production of no _ SNOSN _ (TNF- 伪) PGE2 in serum and reducing the inflammatory infiltration of nerve root tissue.Conclusion Tongluo decoction can relieve pain symptoms of lumbar intervertebral disc herniation effectively, which may be related to alleviating congestion, edema, inflammatory reaction of nerve root tissue, reducing whole blood viscosity, plasma viscosity, prolonging coagulation time, improving blood circulation and so on.
【作者单位】：甘肃中医学院药理教研室;北京中医药大学东直门医院;安福县中医院;中国科学院兰州化学物理研究所;甘肃省中药药理与毒理重点实验室;
【分类号】：R285.5;R-332

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