曲霉多基因系统发育分析及病原性曲霉快速检测方法的建立

发布时间：2018-04-14 21:40

本文选题：曲霉 + 系统发育　；参考：《吉林大学》2009年博士论文

【摘要】： 曲霉与人类的日常生活具有密切关系。有些曲霉在人类生产实践中已被安全应用了近千年,有些曲霉能产生真菌毒素,对农业生产及食品安全造成危害,还有一些曲霉能引起人及动物的侵袭性感染。为了阐明曲霉不同菌种间的系统发育关系及分类地位,澄清不同曲霉之间的亲缘关系与进化路径,本研究对具有重要经济应用价值和临床上常见的共26个种的曲霉进行了系统发育分析。通过对曲霉四种持家基因GAPDH基因、β-tubulin基因、MTO1基因和HSP70基因的编码区部分序列设计兼并引物,对这些基因进行了扩增测序,并提交GenBank。在此基础上对各基因的碱基组成、碱基替换特征及各基因序列间的P距离进行了分析。并采用邻接法(NJ)、非加权分组平均法(UPGMA)法、最大似然法(ML)及最大简约法(MP)对各基因及四种基因的联合序列构建了系统发育树。通过分析,对这26种曲霉的系统发育关系及分类地位进行了阐述,并结合形态学特征对一些菌株进行了重新的鉴定和分类。在以上相关工作基础上,通过对曲霉四种基因测得序列的比对分析,针对病原性烟曲霉、构巢曲霉及黑曲霉的特异位点设计引物及Taqman探针,建立了应用Real-time PCR技术快速检测这三种病原性曲霉的方法。
[Abstract]:Aspergillus has a close relationship with human daily life.Some Aspergillus have been safely used in human production for nearly a thousand years, some Aspergillus can produce mycotoxins, which can cause harm to agricultural production and food safety, and others can cause invasive infection in humans and animals.In order to elucidate the phylogenetic relationship and taxonomic status of Aspergillus sp., the phylogenetic relationship and evolutionary pathway between Aspergillus sp.The phylogenetic analysis of 26 species of Aspergillus sp.The sequence of GAPDH gene, 尾 -tubulin gene, MTO1 gene and HSP70 gene of Aspergillus sp., were amplified and sequenced.On this basis, the base composition, base substitution characteristics and P distance of each gene sequence were analyzed.The phylogenetic tree was constructed by using the adjacent method, the unweighted group average method, the maximum likelihood method (MLL) and the maximum minimization method (MPP) for the combined sequences of each gene and four genes.The phylogenetic relationship and taxonomic status of 26 Aspergillus species were analyzed, and some strains were reidentified and classified according to morphological characteristics.Based on the above work, primer and Taqman probe were designed for the specific sites of Aspergillus fumigatus, Aspergillus Niger and Aspergillus Niger.A method for rapid detection of these three pathogenic aspergillus by Real-time PCR was established.
【学位授予单位】：吉林大学
【学位级别】：博士
【学位授予年份】：2009
【分类号】：R379;R450

【引证文献】