基于NMR的维吾尔医异常黑胆质型大鼠肝癌模型血清代谢组学研究

发布时间：2018-04-15 13:15

本文选题：异常黑胆质 + 肝癌模型　；参考：《中华中医药杂志》2015年10期

【摘要】：依托核磁共振氢谱(1H-NMR)技术和异常黑胆质型大鼠肝癌模型,探讨异常黑胆质性肝癌病证大鼠血清代谢组的变化差异及其机制。方法:根据维吾尔医学理论,建立维吾尔医学异常黑胆质型病证模型,采用二乙基亚硝胺(DEN)诱导20周进一步建立异常黑胆质型肝癌病证大鼠模型。对其血清进行NMR氢谱检测,分段积分后采用OPLS-DA对NMR谱数据进行模式识别分析。结果:与正常对照组比较,在异常黑胆质肝癌模型组大鼠的血清中丙氨酸、糖蛋白、柠檬酸、瓜氨酸、酪氨酸、苯丙氨酸、甲酸含量上升,而α-葡萄糖和β-葡萄糖的含量下降(P0.05);与对照肝癌组相比,异常黑胆质证肝癌模型组大鼠血清中柠檬酸升高与葡萄糖的下降尤为明显(P0.05)。结论:异常黑胆质证肝癌的发生进程中,氨基酸代谢紊乱,能量代谢加剧,脂肪动员增强,糖酵解增加导致乳酸堆积以及肝脏对脂类的肝外转运能力下降,导致血清中丙氨酸、糖蛋白、柠檬酸、酪氨酸、苯丙氨酸、甲酸含量上升,而α-葡萄糖和β-葡萄糖的含量下降,这些可能是异常黑胆质肝癌的潜在代谢标志物。
[Abstract]:Based on 1H-NMR1H-NMR technique and the model of abnormally black bile carcinoma in rats, the changes of serum metabolism and its mechanism in rats with abnormal black bile carcinoma syndrome were studied.Methods: according to the theory of Uygur medicine, the model of abnormal black gallbladder syndrome in Uygur medicine was established, and the model of abnormal black bile carcinoma was further established by diethylnitrosamine (DEN) for 20 weeks.The serum was detected by NMR hydrogen spectrum and OPLS-DA was used to analyze the NMR spectrum data.Results: compared with normal control group, the contents of alanine, glycoprotein, citric acid, citric acid, tyrosine, phenylalanine and formic acid in serum of rats with abnormal black bile carcinoma were increased.The contents of 伪 -glucose and 尾 -glucose decreased P0.05, and the serum citric acid and glucose decreased significantly in the model group of abnormal black gallbladder syndrome compared with the control group.Conclusion: during the development of liver cancer with abnormal black gallbladder syndrome, amino acid metabolism disorder, energy metabolism aggravation, fat mobilization increase, glycolysis increase, lactic acid accumulation and hepatic extrahepatic transport capacity of lipids decrease, and alanine in serum is decreased.The contents of glycoprotein, citric acid, tyrosine, phenylalanine and formic acid increased, while the levels of 伪 -glucose and 尾 -glucose decreased. These may be potential metabolic markers of abnormal black bile carcinoma.
【作者单位】：新疆医科大学基础学院;新疆医科大学新疆地方病分子生物学重点实验室;新疆医科大学;新疆医科大学维吾尔医药系;
【基金】：国际合作基金项目国家自然科学基金项目(No.87E2A0313397,No.81160545)~~
【分类号】：R29;R-332

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