一种简单的分离、培养及鉴定小鼠外周血单核巨噬细胞方法的建立

发布时间：2018-04-16 03:25

本文选题：小鼠 + 外周血单核巨噬细胞　；参考：《首都医科大学学报》2015年04期

【摘要】：目的在L929条件培养基的作用下体外分离、培养小鼠外周血单核巨噬细胞,并进行鉴定。方法密度梯度离心法分离小鼠外周血单核巨噬细胞,用L929条件培养基培养7 d。采用免疫荧光的方法检测F4/80的表达以及细胞的吞噬作用,用流式细胞术进一步检测细胞吞噬率。结果用L929条件培养基诱导培养7 d后小鼠外周血单核细胞表达F4/80,加入细胞吞噬珠之后,在不同时间点用荧光显微镜可以看到红色荧光颗粒聚集在小鼠外周血单核巨噬细胞核周围,用流式细胞仪检测细胞的吞噬率为24.8%±0.79%。结论该方法是一种简单、易操作的体外分离培养和鉴定小鼠外周血单核巨噬细胞的方法。
[Abstract]:Objective to isolate and identify murine peripheral blood mononuclear macrophages in L929 conditioned medium.Methods Mouse peripheral blood mononuclear macrophages were isolated by density gradient centrifugation and cultured on L929 conditioned medium for 7 days.The expression of F4 / 80 and the phagocytosis of F4 / 80 were detected by immunofluorescence, and the phagocytosis rate was further detected by flow cytometry.Results the peripheral blood monocytes of mice were induced to express F4 / 80 in L929 conditioned medium for 7 days, and the cells were added to phagocytic beads.At different time points, red fluorescent particles gathered around the nucleus of mononuclear macrophages in peripheral blood of mice, and the phagocytosis rate of the cells detected by flow cytometry was 24.8% 卤0.79.Conclusion this method is a simple and easy to operate method for isolation, culture and identification of mouse peripheral blood mononuclear macrophages.
【作者单位】：首都医科大学细胞生物学系肝脏保护与再生调节北京市重点实验室;
【基金】：国家自然科学基金(81430013) 北京市属高等学校创新团队建设与教师职业发展计划项目(IDHT20150502)~~
【分类号】：R392

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