骨组织内降钙素基因相关肽（CGRP）免疫阳性神经在骨重建过程中的作用

发布时间：2018-04-16 07:52

本文选题：降钙素基因相关肽 + 骨重建　；参考：《吉林大学》2010年博士论文

【摘要】： 骨组织存在神经分布目前已被证实,但骨组织内神经的功能研究尚处于初级阶段。各种对骨的生理、病理过程的论述中也极少提及神经系统的作用。但临床上观察到的一些现象,如Charcot关节、异位骨化、假体松动、骨质疏松、肥胖相关的骨密度增加,都被发现有神经系统参与的迹象。我们也观察到患有神经纤维瘤病的患者有的表现为骨形成过度,如巨肢症,而有的表现为骨形成不良,如先天性胫骨假关节。这说明不同的神经瘤类型可能是导致患者不同表型的原因。所有这些都提示我们骨骼与神经系统存在内在联系。骨重建是骨骼的最重要的生理过程,骨重建过程是上述病理生理过程最终导致骨质结构改变的“执行者”。因此,研究神经系统在骨组织中的作用自然要着重研究其对骨重建过程的影响。这就是我们立题的出发点。降钙素基因相关肽(CGRP)是目前已知与成骨关系最密切、在骨组织中分布最为广泛的一种神经肽。在体内,CGRP对骨重建的影响,目前存在两种不同的看法,一种是CGRP促进骨形成,另一种看法认为在体内CGRP不具有促进骨形成作用,双方各有各自的实验证据。本研究通过在兔胫骨髓腔内植入形状记忆合金条,从内部对兔胫骨施加一个弯曲应力,建立了一个弯曲应力刺激下的骨重建模型,同时考察了骨重建与CGRP在骨重建局部表达的相互关系,发现在骨内CGRP表达与骨重建呈显著正相关,说明CGRP免疫阳性神经纤维参与体内骨重建过程,进而也说明神经系统对骨重建存在调控作用。本研究创新点:①成功建立了兔胫骨在长期弯曲应力刺激下的骨干皮质骨骨重建模型,证实骨重建过程中骨形成的量与所承受的应力呈正相关。②本研究证实在兔胫骨皮质骨重建过程中CGRP促进骨形成。③本研究提示神经系统参与骨重建调节,CGRP免疫阳性神经纤维是神经系统调控骨重建过程的重要媒介之一。
[Abstract]:The distribution of nerve in bone tissue has been confirmed, but the function of nerve in bone tissue is still in the primary stage.The role of the nervous system is rarely mentioned in the discussion of the physiological and pathological processes of bone.But some clinical phenomena, such as Charcot joint, ectopic ossification, loosening of prosthesis, osteoporosis, increased bone mineral density associated with obesity, have been found to be involved in the nervous system.We also observed that some patients with neurofibromatosis presented with hyperosteogenesis, such as macrosomia, and others with poor bone formation, such as congenital pseudarthrosis of the tibia.This suggests that different types of neuroma may be responsible for different phenotypes of patients.All this suggests that our bones are intrinsically linked to the nervous system.Bone reconstruction is the most important physiological process of bone.Therefore, the study of the role of nervous system in bone tissue should focus on its impact on bone reconstruction process.This is the starting point of our problem.Calcitonin gene-related peptide (CGRP) is known to be the most closely related to osteogenesis and most widely distributed in bone tissue.There are two different views on the effect of CGRP on bone reconstruction in vivo, one is that CGRP promotes bone formation, the other is that CGRP does not promote bone formation in vivo, and each side has its own experimental evidence.In this study, a bone reconstruction model was established by inserting a shape memory alloy strip into the rabbit tibial medullary cavity and applying a bending stress to the rabbit tibia from the inside.At the same time, the relationship between bone reconstruction and local expression of CGRP in bone reconstruction was investigated. It was found that the expression of CGRP in bone was positively correlated with bone reconstruction, indicating that CGRP immunoreactive nerve fibers were involved in the process of bone reconstruction in vivo.It also shows that the nervous system has a regulatory effect on bone reconstruction.In this study, the model of cortical bone reconstruction of the rabbit tibia under long-term bending stress was successfully established by the innovative point: 1.This study confirms that CGRP promotes bone formation in rabbit tibia cortical bone reconstruction. 3. This study suggests that the nervous system is involved in the regulation of bone remodeling. 2. This study suggests that the nervous system participates in the regulation of bone remodeling.The positive nerve fiber is one of the important mediums of nervous system regulating bone reconstruction.
【学位授予单位】：吉林大学
【学位级别】：博士
【学位授予年份】：2010
【分类号】：R392.1

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