江西省鼠类携带汉坦病毒基因特征研究

发布时间：2018-04-16 10:13

本文选题：汉坦病毒 + 基因分型　；参考：《中国媒介生物学及控制杂志》2015年05期

【摘要】：目的了解江西省鼠类汉坦病毒感染情况,分析汉坦病毒基因型别和基因特征。方法于2011年春秋季,在江西省高安市、安义县、新建县、上饶县、上高县、浮梁县室内外以夹夜法捕鼠取其肺,冷冻切片后采用直接免疫荧光法检测鼠肺中汉坦病毒抗原,提取阳性鼠肺标本RNA,采用特异性分型引物进行RT-PCR扩增,确定病毒型别;扩增汉坦病毒全S片段及部分M片段进行序列测定和基因分析。结果共捕获鼠类616只,41份免疫荧光阳性,鼠类汉坦病毒携带率为6.66%;对32份免疫荧光阳性鼠肺标本进行RT-PCR检测,其中10份为汉城型(SEOV型),13份为汉滩型(HTNV型),有9份标本未能分型;对7份标本序列测定和基因分析,结果显示4株HTNV型,3株病毒属SEOV型。结论江西地区鼠类携带汉坦病毒的型别主要为HTNV型和SEOV型,可能存在新亚型的汉坦病毒。
[Abstract]:Objective to investigate the infection of rat Hantavirus in Jiangxi province and to analyze the gene types and characteristics of Hantavirus.Methods in the spring and autumn of 2011, the lungs of Gaoan, Anyi, Xinjian, Shangrao, Shanggao and Fuliang counties in Jiangxi Province were collected by night-clamping method. After frozen sections, the antigen of Hantavirus in the lungs of mice was detected by direct immunofluorescence assay.The positive rat lung samples were extracted and the specific primers were used for RT-PCR amplification to determine the type of the virus. The whole S fragment and some M fragments of Hantavirus were sequenced and analyzed.Results A total of 616 rodents were captured and 41 of them were immunofluorescence positive, and the carrying rate of Hantavirus was 6.660.Among the 32 lung specimens of immunofluorescence positive mice detected by RT-PCR, 10 of them were of Seoul type, 13 of them were Hantan type of HTNV, and 9 of them could not be typed.The results of sequencing and gene analysis of 7 specimens showed that 4 strains of HTNV type and 3 strains of virus belonged to SEOV type.Conclusion the main types of Hantavirus in rats in Jiangxi are HTNV and SEOV, and there may be a new subtype of Hantavirus.
【作者单位】：江西省疾病预防控制中心疾病检测所;
【基金】：江西省卫生厅科技计划项目(20123156)~~
【分类号】：R373.32

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