慢性束缚应激模型对小鼠社交行为的影响

发布时间：2018-04-16 22:13

本文选题：慢性束缚 + 应激　；参考：《中国比较医学杂志》2015年03期

【摘要】：目的探讨慢性束缚应激模型对小鼠社交行为的影响,为应激所致抑郁模型的社会行为学评价方法提供数据支撑。方法采用慢性束缚加孤养方法建立应激所致抑郁模型,模型组每日束缚4 h(10:00~14:00),连续进行35d,单笼饲养。应用糖水偏爱测试确定模型成功,应用社会趋避实验评价慢性束缚应激所致抑郁模型对小鼠社交行为的影响。结果糖水偏爱实验结果显示,慢性束缚组糖水偏爱指数明显下降,与正常组比较具有显著性差异(P0.01)。社会趋避实验结果显示,正常组对刺激鼠笼的嗅闻时间及频次多于空鼠笼,并具有显著性差异(P0.05,P0.01);慢性束缚组对刺激鼠笼和空鼠笼的嗅闻时间及频次相比,无显著性差异。慢性束缚组嗅闻刺激鼠笼时间及频次明显少于正常组,二者相比具有显著性差异(P0.05,P0.01)。慢性束缚组动物第一次嗅闻刺激鼠笼的潜伏期明显比正常组长,二者相比具有极显著性差异(P0.01)。慢性束缚组对刺激鼠笼的偏好指数明显小于正常组,二者具有极显著性差异(P0.01)。结论慢性束缚应激小鼠社交回避的行为学表现,为应激所致抑郁模型社会行为学评价体系的建立提供数据支撑。
[Abstract]:Objective to investigate the effect of chronic restraint stress model on social behavior in mice, and to provide data support for social behavior evaluation of stress induced depression model.Methods the model of depression induced by stress was established by chronic restraint and solitary rearing. The model group was fed with 10: 00: 14: 00 for 35 days.The success of the model was confirmed by sugar water preference test, and the effect of depression induced by chronic restraint stress on social behavior in mice was evaluated by social avoidance test.Results the results of sugar water preference test showed that the index of sugar water preference in the chronic bound group was significantly lower than that in the normal group (P 0.01).The results of social avoidance experiment showed that the time and frequency of sniffing and smelling in the normal group was more than that in the empty cage, and there was a significant difference in the time and frequency of sniffing and smelling in the chronic restraint group, but there was no significant difference in the time and frequency of the stimulation cage and the empty cage in the chronic restraint group.The time and frequency of olfactory and smell stimulation in chronic restraint group was significantly less than that in normal group, and there was significant difference between them.The incubation period of the first time sniffing and smelling stimulation in the chronic bound group was significantly higher than that of the normal group, and there was a significant difference between the two groups (P 0.01).The preference index of the chronic restraint group to the stimulation cage was significantly lower than that of the normal group, and there was a significant difference between the two groups (P 0.01).Conclusion the behavioral manifestations of social avoidance in mice with chronic restraint stress provide data support for the establishment of social behavior evaluation system in stress-induced depression model.
【作者单位】：北京协和医学院比较医学中心中国医学科学院北京协和医学院实验动物研究所卫生部人类疾病比较医学重点实验室国家中医药管理局人类动物模型三级实验室;
【基金】：北京市自然科学基金资助项目(7142105)
【分类号】：R749.4;R-332

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