Annexin-1对小鼠巨噬细胞的功能影响及机制研究

发布时间：2018-04-17 08:25

本文选题：ANXA1 + 活性氧　；参考：《华中科技大学》2009年博士论文

【摘要】： 外源性Annexin-1抑制脂多糖诱导的小鼠巨噬细胞增值和活性氧产生部分通过CRAC通道调节但与NF-κB通路无关目的：研究外源性Annexin-l(ANXAl)对脂多糖诱导的小鼠巨噬细胞RAW264．7增殖，活性氧的产生，钙信号转导及NF-κB的活化的影响并探讨其可能机制。方法：体外培养小鼠巨噬细胞RAW264．7细胞，，分为空白组，脂多糖组，ANXAl处理组。CCK-8法检测巨噬细胞的增殖能力，流式细胞仪，荧光显微镜检测活性氧的生成水平，激光共聚焦显微镜检测活细胞内游离钙离子浓度的变化，Western blot检测IκBα降解和NF-κB的转位情况。结果：(1)外源性Annexin-1剂量依赖性地抑制LPS诱导的小鼠RAW264．7巨噬细胞的增殖和活性氧的生成；外源性Annexin-l对LPS诱导的活性氧生成的抑制效应随作用时间而变化。(2)LPS通过开放CRAC通道增加[Ca~(2+)]_i，外源性Annexin-1剂量依赖性地抑制LPS诱导的[Ca~(2+)]_i的增加。(3)LPS诱导的小鼠RAW264．7巨噬细胞的增殖和活性氧的生成与CRAC通道相关。(4)外源性Annexin-1不影响LPS诱导的IκB的降解和NF-κB的转位。结论：外源性Annexin-1抑制LPS诱导的小鼠RAW264．7巨噬细胞的增殖和活性氧的生成可能部分通过CRAC通道调节但与NF-κB通路无关。
[Abstract]:Exogenous Annexin-1 inhibits lipopolysaccharide-induced macrophage proliferation and reactive oxygen species production via CRAC channel but not NF- 魏 B pathwayAim: to investigate the effects of exogenous Annexin-lANGXAlon on RAW264.7 proliferation, reactive oxygen species production, calcium signal transduction and NF- 魏 B activation in murine macrophages induced by lipopolysaccharide (LPS).Methods: murine macrophages RAW264.7 cells were cultured in vitro and divided into blank group, lipopolysaccharide group, ANXAl treatment group. CCK-8 method was used to detect the proliferation of macrophages, flow cytometry and fluorescence microscopy were used to detect the level of reactive oxygen species (Ros) production.The concentration of free calcium in living cells was detected by laser confocal microscopy. The degradation of I 魏 B 伪 and the translocation of NF- 魏 B were detected by Western blot.Results Annexin-1 inhibited the proliferation of RAW264.7 macrophages induced by LPS and the production of reactive oxygen species (Ros) in a dose-dependent manner.The inhibitory effect of exogenous Annexin-l on the production of reactive oxygen species induced by LPS varies with time. LPs increases [Ca~(2] Ca~(2 through open CRAC channels, and exogenous Annexin-1 dose-dependently inhibits the increase of [Ca~(2] I induced by LPS. LPS-induced RAW264.7 macrophages in miceThe proliferation of cells and the production of reactive oxygen species (Ros) were related to CRAC channels. (4) exogenous Annexin-1 did not affect the degradation of I 魏 B and the translocation of NF- 魏 B induced by LPS.Conclusion: exogenous Annexin-1 inhibits the proliferation of RAW264.7 macrophages induced by LPS and the production of reactive oxygen species, which may be partly regulated by CRAC channel but not related to NF- 魏 B pathway.
【学位授予单位】：华中科技大学
【学位级别】：博士
【学位授予年份】：2009
【分类号】：R363

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