EX527对热量限制保护SH-SY5Y细胞的影响

发布时间：2018-04-17 15:27

本文选题：热量限制 + SIRT抑制剂　；参考：《首都医科大学学报》2015年05期

【摘要】：目的观察SIRT1抑制剂EX527对热量限制处理的人神经母细胞瘤株SH-SY5Y细胞的影响。方法体外培养SHSY5Y细胞,分为正常对照组(NC组)、热量限制组(CR组)和热量限制加SIRT1抑制剂组(CR+EX527组),光镜下观察细胞形态学改变,测定各组细胞噻唑蓝(thiazolyl blue tetrazolium bromide,MTT)代谢率及乳酸脱氢酶(lactate dehydrogenase,LDH)漏出率,应用蛋白免疫印迹(Western blotting)法测定SIRT1表达量。结果与NC组相比,CR组细胞形态好,表现为细胞胞体饱满、突起伸展、贴壁牢固,MTT代谢率升高(P0.05),LDH漏出率有降低趋势,CR组和CR+EX527组SIRT1表达均增加(P0.05);与CR组相比,CR+EX527组细胞形态较差,突起回缩,贴壁性不好,MTT代谢率降低(P0.05),LDH漏出率有升高趋势,SIRT1表达略减少。结论热量限制对神经细胞具有保护作用,而SIRT1抑制剂EX527能够减弱热量限制的神经保护作用,提示热量限制对SH-SY5Y细胞的保护作用可能是由SIRT1介导的。
[Abstract]:Objective to observe the effect of SIRT1 inhibitor EX527 on human neuroblastoma cell line SH-SY5Y treated with heat restriction.Methods SHSY5Y cells were cultured in vitro and divided into normal control group (NC group), caloric restriction group (CR group) and caloric restriction plus SIRT1 inhibitor group (CR EX527 group). Morphological changes were observed under light microscope.The metabolic rate of thiazolyl blue tetrazolium bromidede (MTT) and the leakage rate of lactate dehydrogenase (LDH) were measured. The expression of SIRT1 was detected by Western blotting.Results compared with NC group, the cell morphology of CR group was better than that of NC group, which showed that the cell body was plump, the protuberance was extended, the metabolic rate of adherent to the cell wall was increased, the leakage rate of SIRT1 was decreased in CR group and CR EX527 group, the expression of SIRT1 in CR group and CR EX527 group was higher than that in CR group, and the cell morphology in CR EX527 group was worse than that in CR group.Protruding back, MTT metabolic rate decreased, the leakage rate of LDH increased and the expression of SIRT1 decreased slightly.Conclusion caloric restriction has protective effect on nerve cells, while SIRT1 inhibitor EX527 can attenuate the neuroprotective effect of caloric restriction on SH-SY5Y cells, suggesting that the protective effect of caloric restriction on SH-SY5Y cells may be mediated by SIRT1.
【作者单位】：首都医科大学宣武医院中心实验室;北京市老年病医疗研究中心;北京脑重大疾病研究院阿尔茨海默病研究所;神经变性病教育部重点实验室;
【基金】：国家自然科学基金(81472007) 北京市自然科学基金(7132044)~~
【分类号】：R329.2

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