烟曲霉提取物诱导小鼠哮喘模型的建立

发布时间：2018-04-18 16:29

本文选题：烟曲霉提取物 + 哮喘　；参考：《心肺血管病杂志》2015年02期

【摘要】：目的:探讨小鼠哮喘模型建立的方法,为开展哮喘发生机制或药物治疗研究奠定基础。方法:40只,SPF级C57BL/6小鼠,雌雄各20只,随机分成对照组和模型组两组。模型组小鼠采用气管滴注烟曲霉提取物,隔天给药,对照组小鼠采用无菌0.9%氯化钠溶液气管滴注。给药21天后,最后一次给药24小时后收取肺泡灌洗液,离心后灌洗液上清检测炎症因子水平,灌洗细胞做Giemsa染色分类计数;取左肺做AB-PAS染色,右肺提取mRNA;腹主动脉取血ELISA检测Ig E水平。结果:与对照组相比,模型组小鼠可见典型的哮喘症状,肺泡灌洗液中炎症细胞总数、嗜酸性粒细胞和中性粒细胞较对照组显著增多,肺泡灌洗液IL-3、IL-4、IL-5、IL-6及IL-17表达上调,与对照组相比分别为P0.05和P0.01。血清Ig E较对照组明显增加[(23.84±3.65)vs.(6.22±1.06)mg/L,P0.01];AB-PAS染色黏液分泌较对照组明显加重。结论:烟曲霉提取物气管滴注可成功诱导小鼠哮喘模型。
[Abstract]:Objective: to explore the method of establishing mouse asthma model and lay a foundation for the study of asthma mechanism or drug therapy.Methods 40 C57BL/6 mice, 20 male and 20 female, were randomly divided into two groups: control group and model group.The model group mice were treated with tracheal drip of Aspergillus fumigatus extract and the control group mice were treated with aseptic 0.9% sodium chloride solution.After 21 days of administration, the alveolar lavage fluid was collected 24 hours after the last administration, the level of inflammatory factors was detected in the supernatant of the lavage fluid after centrifugation, the Giemsa staining was performed in the lavage cells, and the AB-PAS staining was performed in the left lung.MRNAs were extracted from the right lung and ELISA was taken from the abdominal aorta to detect the level of IgE.Results: compared with the control group, the model group showed typical asthmatic symptoms, the total number of inflammatory cells, eosinophils and neutrophils in the alveolar lavage fluid increased significantly compared with the control group, and the expression of IL-6 and IL-17 in the alveolar lavage fluid was upregulated.Compared with the control group, P0.05 and P0.01, respectively.Compared with the control group, serum IgE increased significantly [23.84 卤1.06 mg / L P0.01] the mucus secretion of AB-PAS staining was significantly higher than that of the control group.Conclusion: trachea drip of Aspergillus fumigatus extract can successfully induce asthma model in mice.
【作者单位】：首都医科大学附属北京安贞医院;北京市心肺血管疾病研究所
【基金】：国家自然科学基金资助项目(81130001;81100021)
【分类号】：R562.25;R-332

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