Axl和Mer受体调控小鼠红细胞分化

发布时间：2018-04-21 05:53

本文选题：Axl + Mer　；参考：《基础医学与临床》2015年12期

【摘要】：目的研究Axl和Mer受体是否参与红细胞分化的调控。方法单基因(AXL-/-和MER-/-)和双基因(AXL-/-MER-/-)敲除小鼠,利用组织切片观察小鼠骨髓、肝脏和脾脏的组织结构;计数小鼠单根股骨内骨髓细胞的数量;用实时定量PCR检测与红细胞分化相关基因mRNA的表达。结果双基因敲除的小鼠单根股骨骨髓内总细胞、单个核细胞和成熟红细胞的数量分别下降了40%、34%和53%(P0.01);骨髓细胞排列稀疏,血窦内、外成熟红细胞数量明显减少;虽然外周血中红细胞数量和血红蛋白的含量没有改变,但存在着明显的髓外造血,肝脏里出现了造血岛,脾脏中红髓明显地增生。而单基因敲除小鼠骨髓中红细胞数量以及组织结构未见改变。且双基因敲除小鼠中红系细胞内GATA-1和EpoR的mRNA表达分别降低了65%和75%(P0.01)。结论 Axl和Mer受体通过GATA-1和EpoR来调节红细胞的分化,并且功能上存在重叠、过剩和代偿。
[Abstract]:Objective to investigate whether Axl and Mer receptors are involved in the regulation of erythrocyte differentiation. Methods single gene (AXL-/- and MER-/-) and double gene (AXL-/-MER-/-) knockout mice were used to observe the tissue structure of bone marrow, liver and spleen of mice by tissue section. The number of bone marrow cells in the single femur of mice was counted, and the differentiation phase of red blood cells was detected by real-time quantitative PCR. The results showed that the number of total cells in bone marrow, mononuclear cells and mature red cells in single root bone marrow of mice with double gene knockout decreased by 40%, 34% and 53% (P0.01), and the number of bone marrow cells was sparse, and the number of mature red cells in the blood sinuses decreased significantly, although the number of red blood cells and the content of hemoglobin in the peripheral blood were not. However, there were obvious extramedullary hematopoiesis, the hematopoietic island in the liver, the red pulp in the spleen were obviously proliferated, and the number of red cells and the structure of the bone marrow in the single gene knockout mice were not changed. The mRNA expression of GATA-1 and EpoR in the erythroid cells in the double gene knockout mice was reduced by 65% and 75% (P0.01). Conclusion Axl and Mer were found. The body regulates the differentiation of red blood cells through GATA-1 and EpoR, and there are overlaps, excess and compensatory functions.

【作者单位】：华北理工大学生命科学学院;
【基金】：河北省自然科学基金(H2013209194)
【分类号】：R3416

【相似文献】