更年期高血压燥证大鼠模型的构建

发布时间：2018-04-21 13:29

本文选题：高血压 + 更年期　；参考：《中国全科医学》2015年30期

【摘要】：目的探索更年期高血压燥证大鼠模型的建立方法。方法 2014年1—3月选取雌性自发性高血压大鼠(SHR)12只,采用摸球法将SHR随机分为更年期高血压组和更年期高血压燥证造模组,每组6只。采用外科手术法摘除两组大鼠双侧卵巢,制备更年期高血压大鼠。对更年期高血压燥证造模组大鼠施加人工燥证环境,饲喂西北燥证食物进行造模。观察大鼠生物学特征(毛色、精神状态、兴奋度、进食量、饮水量、体质量、大小便性状、舌象、爪象等)、血清神经递质〔促肾上腺皮质激素释放激素(CRH)、促肾上腺皮质激素(ACTH)、去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)〕及激素〔雌二醇(E2)、睾酮(T)〕水平和脏器(肝脏、脾脏、肾上腺、下丘脑)组织结构。结果更年期高血压燥证造模组大鼠较更年期高血压组体质量降低,饮水量增加,尿量减少(P0.05)。更年期高血压燥证造模组大鼠血清CRH、NE、DA、5-HT水平较更年期高血压组升高(P0.05)。两组大鼠ACTH水平比较,差异无统计学意义(P0.05)。更年期高血压燥证造模组大鼠较更年期高血压组血清E2、T水平降低,E2/T升高(P0.05)。两组大鼠肝脏、脾脏、肾上腺、下丘脑组织结构均发生明显改变。结论通过对更年期高血压大鼠施加人工燥证环境,给予西北燥证食物,控制饮水量及进食量,可成功建立更年期高血压燥证大鼠模型。
[Abstract]:Objective to explore the method of establishing the rat model of menopausal hypertension dryness syndrome. Methods from January to January 2014, 12 female spontaneously hypertensive rats were selected. SHR was randomly divided into menopausal hypertension group and menopausal hypertension dryness model group with 6 rats in each group. The menopausal hypertensive rats were prepared by surgical excision of bilateral ovaries. The rats of climacteric hypertensive dryness syndrome model group were fed with the food of northwest dryness syndrome. The biological characteristics (color, mental state, excitability, food intake, drinking water, body mass, fecal and urinal characteristics, tongue picture) of rats were observed. The levels of serum neurotransmitters (CRH, ACTH, NE, 5-HT) and hormone (estradiol, estradiol, testosterone) and visceral organs (liver, spleen, adrenal gland, adrenocorticotropic hormone (ACTH), noradrenaline (NE), dopamine (DA), 5-hydroxytryptamine (5-HT)) and the organs (liver, spleen, adrenal gland), and the levels of serum neurotransmitters (estradiol, estradiol, estradiol, testosterone) and organs (liver, spleen, adrenal gland), Hypothalamus). Results compared with the climacteric hypertension group, the body weight, drinking water and urine volume of the climacteric hypertensive dryness model group were lower, and the urine volume decreased than that of the climacteric hypertension group (P 0.05). Compared with climacteric hypertension group, serum CRHNE-DA-5-HT level in model group was higher than that in climacteric hypertension group (P 0.05). There was no significant difference in ACTH level between the two groups (P 0.05). Compared with the climacteric hypertension group, the serum E _ 2 / T level in the climacteric hypertensive dryness model group was lower than that in the climacteric hypertension group. The structure of liver, spleen, adrenal gland and hypothalamus in both groups were obviously changed. Conclusion the rat model of menopausal hypertension dryness syndrome can be successfully established by applying artificial dryness syndrome environment to climacteric hypertensive rats, giving food to northwest dryness syndrome, controlling the quantity of drinking water and eating quantity.
【作者单位】：新疆医科大学附属中医医院高血压科;
【基金】：国家自然科学基金资助项目(81160430)
【分类号】：R-332;R271.116

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