一种新型大鼠固定装置的制作与使用方法

发布时间：2018-04-21 23:33

本文选题：清醒大鼠 + 固定装置　；参考：《中国比较医学杂志》2015年08期

【摘要】：目的制作一种新型的清醒大鼠固定装置,并规范其使用方法,以弥补现有固定方法的不足。确保无论使用者有无经验均能高效完成清醒动物固定及后续操作。方法用透明塑料薄膜,塑料封口机,海绵等材料设计制作大鼠固定装置,利用大鼠的逃匿天性,达到自束缚的效果。并通过固定与注射实验比较该新型方法较传统方法的优点。结果与传统的固定方法相比,使用该新型固定装置不仅更节约时间,其中固定操作平均耗时由18.13 s减少到10.03 s,,提高了44.7%;固定与注射平均耗时从139.33 s减少到52.26 s,提高了55.3%,并能显著降低大鼠应激反应。结论该装置和方法适用于清醒大鼠固定及注射,并能由单人完成尾静脉注射和采血操作,可显著减少操作时间并降低大鼠的应激反应,使实验结果更准确可靠。特别适合缺乏动物实验操作经验的人群,值得在教学和科研中推广。
[Abstract]:Objective to make a new type of fixed device for conscious rats and standardize its use to make up for the deficiency of the existing fixation methods. Ensure that lucid animal fixation and follow-up can be performed efficiently regardless of the user's experience. Methods using transparent plastic film, plastic sealing machine, sponge and other materials to design and manufacture the fixed device of rats, and make use of the nature of escape of rats to achieve the effect of self-binding. The advantages of the new method are compared with that of the traditional method by immobilization and injection experiments. Results compared with the traditional fixation method, the use of the new fixing device not only saves more time, The average time of fixation and injection decreased from 18.13 s to 10.03 sL, and the average time of fixation and injection decreased from 139.33 s to 52.26 s, which increased 55.3 s, and significantly decreased the stress response of rats. Conclusion the device and method are suitable for fixation and injection in conscious rats, and can be used to complete caudal vein injection and blood collection by single person, which can significantly reduce the operating time and stress response of rats, so that the experimental results are more accurate and reliable. It is especially suitable for people who lack experience in animal experiment, and is worth popularizing in teaching and scientific research.
【作者单位】：第三军医大学学员旅14营;
【基金】：国家自然科学基金(编号:31371116)
【分类号】：R-331

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