高脂模型长爪沙鼠生化指标变化

发布时间：2018-04-22 01:37

本文选题：疾病模型 + 动物　；参考：《现代医药卫生》2015年24期

【摘要】：目的观察高脂饲料喂养14周的长爪沙鼠生化指标变化。方法选取雄性长爪沙鼠20只,随机分为高脂饲料组和对照组,每组10只。对照组喂养普通饲料,高脂饲料组喂养含1.0%胆固醇、20.0%猪油的高脂饲料。14周后颌下静脉丛采血,检测血糖(GLU)、三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)等。结果高脂饲料组长爪沙鼠血清TC、LDL-C、TG、HDL-C水平[分别为(14.27±2.97)、(4.72±1.94)、(3.04±1.22)、(3.43±0.90)mmol/L]明显高于对照组[分别为(1.72±0.22)、(0.19±0.09)、(1.53±0.49)、(1.46±0.25)mmol/L],差异均有统计学意义(P0.01)。GLU水平[(6.47±0.55)mmol/L]低于对照组[(8.19±1.02)mmol/L],但差异无统计学意义(P0.05)。结论高脂饲料喂养能明显升高长爪沙鼠血脂水平,导致长爪沙鼠高脂血症。
[Abstract]:Objective to observe the changes of biochemical indexes in gerbils with high fat diet for 14 weeks. Methods Twenty male gerbils were randomly divided into high fat diet group and control group with 10 rats in each group. The control group was fed with normal diet, the high fat diet containing 1.0% cholesterol and 20.0% lard was fed with high fat diet for 14 weeks, blood was collected from the submaxillary venous plexus. Blood glucose, triglyceride, total cholesterol, high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) were detected. Results the level of HDL-C in serum of the rats with high fat feed group was significantly higher than that of the control group (4.72 卤1.94 卤1.94 卤3.43 卤0.90)mmol/L, respectively, = 1.72 卤2.97 卤4.72 卤1.22), but the difference was not statistically significant (1.72 卤0.22) 卤(0.19 卤0.09) 卤(1.46 卤0.25)mmol/L). The difference was statistically significant (P < 0.01). GLU [6.47 卤0.55)mmol/L] was significantly lower than that in the control group [[8.19 卤1.02)mmol/L]], but there was no significant difference between the two groups (P < 0.05). Conclusion High fat diet can significantly increase the level of blood lipid in gerbils and lead to hyperlipidemia.
【作者单位】：广东医学院生物化学与分子生物学教研室;东莞市慢性病防治院检验科;
【基金】：广东省高等学校人才引进专项资金资助项目(粤财教[2013]246号)
【分类号】：R589.2;R-332

【参考文献】