慢性强迫游泳对大鼠行为学及脑内PSD-95表达的影响

发布时间：2018-04-22 17:52

本文选题：慢性强迫游泳 + 海马　；参考：《福建医科大学》2010年硕士论文

【摘要】： 目的:1、成功建立慢性强迫游泳大鼠动物模型; 2、观察慢性强迫游泳对大鼠生物、行为、心理的影响; 3、观察慢性强迫游泳对大鼠脑内海马、前额叶皮质、基底节PSD-95表达的影响。 4、给予MK801干预,观察其作用,并探讨其机制。方法:建立慢性强迫游泳大鼠动物模型。动物按体重随机分组,分为对照组(C),单纯应激组(M),干预组(T)。强迫游泳的第1、6、10、14天测量大鼠体重;第4周行糖水消耗实验,观察大鼠3小时1%糖水和饮用水的消耗量;采用相对消耗量评定不同体重大鼠的液体消耗;计算每只大鼠的不动时间,并且以不动时间作为行为学指标;运用Western Blot法检测各组大鼠脑内海马、前额叶皮质、基底节各区PSD-95的表达与变化。结果:(1)强迫游泳后,与对照组比较,第14天大鼠体重增长量降低,蔗糖溶液消耗量以及总液体消耗量降低明显;不动时间第1至4天无显著性变化,第5至14天明显增长;第2至14天不动时间明显长于第1天;海马区、前额区和基底节区的PSD-95量显著增加。 (2)注射MK801干预后,与单纯应激组比较,第14天大鼠体重增长量升高,蔗糖溶液消耗量以及总液体消耗量增加明显;不动时间第1至4天无显著性差异,第5至14天明显缩短;第2至6天、第8、10天不动时间均明显长于第1天;其余天数与第1天比较无明显差别;海马区、前额区和基底节区的PSD-95量显著下降。结论:慢性强迫游泳能够成功的建立作为慢性应激、抑郁症的动物模型。慢性强迫游泳能够使大鼠的体重增长减慢、快感缺乏、无助感出现、自主活动减少。慢性强迫游泳后脑内海马、前额叶皮质、基底节的PSD-95表达发生了变化,推测PSD-95和慢性强迫游泳后出现的生物、行为、心理改变有关;MK801能够逆转这种情况,可能和MK801能够与谷氨酸发生拮抗作用,避免神经元过度兴奋有关。
[Abstract]:Objective to establish a rat model of chronic forced swimming successfully. 2. The effects of chronic forced swimming on biology, behavior and psychology of rats were observed. 3. To observe the effect of chronic forced swimming on PSD-95 expression in hippocampus, prefrontal cortex and basal ganglia of rats. 4. MK801 intervention was given to observe its effect and explore its mechanism. Methods: the animal model of chronic forced swimming was established. Animals were randomly divided into two groups according to their body weight: control group, control group, stress group and intervention group. The rats' body weight was measured at the 1st day of forced swimming, and the consumption of 1% sugar water and drinking water was observed at the 4th week, and the liquid consumption of rats of different body weight was evaluated by relative consumption. Western Blot method was used to detect the expression and change of PSD-95 in hippocampus, prefrontal cortex and basal ganglia of rats. Results (1) after forced swimming, the body weight increased significantly, the consumption of sucrose solution and the consumption of total fluid decreased significantly on the 14th day compared with the control group, but the immobility time did not change significantly on the 1st to 4th day, but increased significantly on the 5th to 14th day. The time of immobility from day 2 to day 14 was longer than that of day 1, and the amount of PSD-95 in hippocampus, prefrontal region and basal ganglia increased significantly. (2) compared with the control group, the body weight increased, the sucrose solution consumption and the total fluid consumption increased significantly on the 14th day, but the immobility time was not significantly different from the 1st to 4th day, and the 5th to 14th day was significantly shorter than that in the simple stress group. From day 2 to day 6, the immobility time of day 810 was longer than that of day 1, but the other days were not significantly different from that of day 1. The amount of PSD-95 in hippocampal region, prefrontal area and basal ganglia decreased significantly. Conclusion: chronic forced swimming can be used as an animal model of chronic stress and depression. Chronic forced swimming can slow down weight gain, lack of pleasure, helplessness, and decrease in autonomous activities. The expression of PSD-95 in hippocampus, prefrontal cortex and basal ganglia after chronic forced swimming has changed. It is speculated that PSD-95 and the biological, behavioral and psychological changes after forced swimming are related to the reverse effect of MK801. It may be related to the antagonistic effect of MK801 on glutamate and to avoid neuronal hyperstimulation.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R-332

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