芳香精油通过嗅觉通路调节大鼠血压的实验研究

发布时间：2018-04-23 10:50

本文选题：精油 + 嗅觉通路　；参考：《安徽医科大学》2009年硕士论文

【摘要】： 目的通过大鼠吸入3种精油来观察其对血压的影响,并结合嗅上皮毁损模型,以及免疫组织化学方法检测不同脑区的神经递质变化,探讨精油调节血压的可能机制,寻找新的降低血压的药物和作用途径,为临床治疗高血压病提供实验依据。方法本研究首先将大鼠随机分为空白组、薰衣草精油组、迷迭香精油组和丁香精油组,采用MedLab 6.0生物信号采集处理系统测定大鼠血压,通过实验筛选出具有降压作用的薰衣草精油。再取大鼠,随机分为空白组、薰衣草精油组、假手术+薰衣草精油组和模型(嗅上皮毁损)+薰衣草精油组,其中嗅上皮毁损模型采用硫酸锌(ZnSO4)制备。利用MedLab 6.0生物信号采集处理系统测定大鼠血压,并采用酶联免疫吸附法测定外周血儿茶酚胺(CA)含量;通过免疫组织化学方法检测各组大鼠脑干、下丘脑、杏仁核等脑区的γ-氨基丁酸(GABA)、5-羟色胺(5-HT)、去甲肾上腺素(NE)神经递质变化。结果 1.薰衣草精油组、迷迭香精油组、丁香精油组和空白组4组间SP、DP、MAP的差异均有显著性。空白组在吸入蒸馏水结束后100 min内SP、DP、MAP无明显变化,差异无显著性。与空白组比较,薰衣草精油组SP、DP、MAP降低,在薰衣草精油吸入结束后80~100 min时降低最明显,差异有显著性,在观察期100 min内血压未回复到干预前水平。而迷迭香精油组、丁香精油组SP、DP、MAP与空白组比较,差异无显著性。与薰衣草精油组比较,迷迭香精油组、丁香精油组SP、DP、MAP无明显降低,其差异具有显著性。迷迭香精油组和丁香精油组SP、DP、MAP相比差异无统计学意义。 2.空白组、薰衣草精油组、假手术+薰衣草精油组和模型(嗅上皮毁损)+薰衣草精油组4组比较,4组间SP、DP、MAP差异有显著性。与空白组比较,薰衣草精油组、假手术+薰衣草精油组SP、DP、MAP均降低,在吸入精油结束后80~100 min差异有显著性;而模型+薰衣草精油组SP、DP、MAP无明显降低,差异无显著性。与模型+薰衣草精油组相比,假手术+薰衣草精油组SP、DP、MAP降低,在吸入精油结束后80~100 min差异有显著性。薰衣草精油组、假手术+薰衣草精油组SP、DP、MAP相比差异无统计学意义。 3.与吸入前比较,薰衣草精油组在吸入结束后100 min血清CA浓度明显降低,其差异具有统计学意义;而空白组、模型+薰衣草精油组在吸入前后血清CA浓度均无明显变化,差异无显著性。吸入前,各组血清CA浓度差异无统计学意义。吸入后,与空白组比较,薰衣草精油组血清CA浓度降低,差异有显著性;而模型+薰衣草精油组血清CA浓度差异无统计学意义。 4.免疫组织化学结果显示,与空白组比较,薰衣草精油组大鼠的杏仁核、下丘脑、脑干核团内5-HT、GABA表达增加,而NE表达减少。模型+薰衣草精油组与空白组比较,5-HT、GABA、NE的表达无明显差异。结论 1.大鼠吸入薰衣草精油能降低SP、DP、MAP; 2.薰衣草精油通过嗅觉途径降低大鼠SP、DP、MAP; 3.大鼠吸入薰衣草精油降低外周血儿茶酚胺含量可能是其降低血压的外周作用机制; 4.薰衣草精油通过嗅觉通路引起大鼠脑内GABA、5-HT、NE神经递质的改变可能是其降低血压的中枢作用机制。
[Abstract]:objective
The effects of 3 kinds of essential oils on blood pressure were observed in rats, and the changes of neurotransmitters in different brain regions were detected in combination with the damage model of olfactory epithelium and immunohistochemical method. The possible mechanism of essential oil regulating blood pressure was explored, and a new drug and path to reduce blood pressure were found, and the experimental basis for clinical treatment of hypertension was provided.
Method
In this study, rats were randomly divided into blank group, lavender essential oil group, rosemary essential oil group and lilac essential oil group. The MedLab 6 biological signal collection and treatment system was used to determine the blood pressure of rats. The experiment was used to select lavender essential oil with antihypertensive effect. Then the rats were randomly divided into blank group, lavender essential oil group, sham operation + lavender. Essential oil group and model (olfactory epithelium damage) + lavender essential oil group, of which the olfactory epithelium damage model was prepared by zinc sulfate (ZnSO4). The blood pressure of rats was measured by MedLab 6 biological signal acquisition and treatment system, and the content of catecholamine (CA) in peripheral blood was measured by enzyme linked immunosorbent assay, and the brainstem of each group was detected by immunohistochemistry. The changes of GABA, 5-, 5-HT and NE in the hypothalamus, amygdala and other brain regions.
Result
1. the SP, DP, MAP of lavender essential oil group, rosemary essential oil group, lilac essential oil group and blank group have significant difference. There is no significant change in SP, DP, MAP in 100 min after the end of distilled water. Compared with the blank group, lavender essential oil group SP, DP, MAP decrease, and 80~100 min after lavender essential oil is inhaled at the end of the lavender essential oil. The difference was significant. The blood pressure did not return to the pre intervention level in the 100 min observation period. There was no significant difference in the difference between the rosemary oil group and the SP, DP and MAP in the lilac essential oil group and the lavender essential oil group, and the Rosmarinus essential oil group, the SP, DP, and MAP in the lilac essential oil group were not significantly reduced, and the difference was significant. Rosemary essence was significant. There was no significant difference in SP, DP and MAP between oil group and clove essential oil group.
2. blank group, lavender essential oil group, sham operation + lavender essential oil group and model (olfactory epithelium damage) + lavender essential oil group 4 groups, the difference between the 4 groups SP, DP, MAP was significant. Compared with the blank group, lavender essential oil group, sham operation + lavender essential oil group SP, DP, MAP decreased, and the difference of 80~100 min after the end of the essential oil was significant; and model + The SP, DP, MAP of lavender essential oil group did not decrease significantly, and the difference was not significant. Compared with the model + lavender essential oil group, the sham operation + lavender essential oil group SP, DP, MAP decreased, and the difference in 80~100 min after the end of the essential oil was significant. Lavender essential oil group, sham operation + lavender essential oil group had no statistical significance compared with SP, DP, MAP.
3. compared with before inhalation, the concentration of serum CA in the lavender essential oil group decreased significantly at the end of the inhalation of 100 min, and the difference was statistically significant. But in the blank group, there was no significant change in serum CA concentration in the model + lavender essential oil group before and after inhalation. There was no significant difference in the concentration of serum CA before inhalation. After inhalation, the concentration of serum was not statistically significant. Compared with white group, the concentration of serum CA in lavender essential oil decreased, but there was no significant difference in serum CA concentration in model + lavender essential oil group.
4. immunohistochemical results showed that, compared with the blank group, the expression of 5-HT, GABA in the amygdala, hypothalamus and brainstem nuclei increased in the amygdala, hypothalamus and brainstem nuclei of lavender group. The expression of 5-HT, GABA and NE was not significantly different from that of the model + lavender essential oil group and the blank group.
conclusion
The 1. rats inhaled lavender essential oil could reduce SP, DP and MAP.
2. lavender essential oil reduces SP, DP and MAP in rats by olfactory pathway.
3. the decrease of catecholamine in peripheral blood by inhaling lavender essential oil may be the peripheral mechanism of lowering blood pressure.
4. the change of GABA, 5-HT and NE neurotransmitters in rat brain induced by olfactory pathway may be the central mechanism of lavender essential oil to reduce blood pressure.

【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R338.2

【参考文献】