大鼠去神经支配脾脏内淋巴细胞ERK表达变化的研究

发布时间：2018-04-24 03:15

本文选题：去神经 + 脾脏　；参考：《现代生物医学进展》2010年10期

【摘要】：目的:探讨脾脏神经支配与免疫功能关系。方法:清洁级成年雄性SD大鼠20只,随机分成实验组、对照组。手术切除实验组10只大鼠脾脏神经纤维,饲养1周后取出脾脏,4%多聚甲醛固定,石蜡切片行HE染色及ABC免疫组织化学染色。结果:(1)HE染色两组大鼠脾脏结构结构清晰,动脉周围淋巴鞘内淋巴细胞密集成团状,红髓内可见大量红细胞,在去除大鼠脾脏神经支配的实验组较对照组脾脏大体结构无明显变化;(2)两组脾脏淋巴细胞均有ERK表达,对照组正常脾脏表达ERK阳性细胞占16.2%,实验组仅占6.0%。实验组脾脏内表达ERK阳性细胞数量明显低于对照组,差异具有显著性(P0.01),同时ERK表达量也低于对照组,且差异具有显著性(P0.01)。结论:在去除神经支配后,脾脏内淋巴细胞增殖活性明显降低,在一定程度上影响到脾脏免疫功能。
[Abstract]:Objective: To investigate the relationship between spleen innervation and immune function. Methods: 20 adult male SD rats were randomly divided into experimental group and control group. The spleen nerve fibers were removed from 10 rats in the experimental group. After 1 weeks, the spleen was removed, 4% polyformaldehyde was fixed, the paraffin section was stained with HE staining and ABC immunohistochemical staining. The results were (1) HE staining. In the two groups, the structure of the spleen was clear, the lymphocytic lymphocytes in the peripheral lymphatic sheath of the artery were densely integrated, and a large number of red cells were found in the red pulp. In the experimental group, there was no significant change in the general structure of the spleen in the experimental group. (2) the two groups of spleen lymphocytes had ERK expression, and the normal spleen expressed ERK positive cells in the control group. 16.2%, the number of ERK positive cells expressed in the spleen of 6.0%. experimental group was significantly lower than that in the control group. The difference was significant (P0.01), and the expression of ERK was also lower than that of the control group, and the difference was significant (P0.01). Conclusion: after the removal of innervation, the proliferation activity of the spleen cells in the spleen was significantly reduced, and to a certain extent, the spleen affected the spleen. Dirty immune function.

【作者单位】：第四军医大学唐都医院普外科;
【分类号】：R392

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