临床分离表皮葡萄球菌附属基因调节因子agr基因多态性分析

发布时间：2018-04-25 13:34

  本文选题：表皮葡萄球菌 + 附属基因调节因子　； 参考：《中国实验诊断学》2015年04期

【摘要】：目的探讨临床分离表皮葡萄球菌附属基因调节因子agr基因多态性,了解表皮葡萄球菌的分子特征。方法收集84株临床分离并经过全自动微生物鉴定系统准确鉴定的表皮葡萄球菌,通过PCR扩增esp和mecA基因准确鉴定和区分甲氧西林敏感表皮葡萄球菌(MSSE)和耐甲氧西林表皮葡萄球菌(MRSE),采用多重PCR扩增表皮葡萄球菌的agr等位基因,分析agr基因多态性。结果 84株临床分离表皮葡萄球菌中,20株为MSSE,占23.80%,其中agrⅠ型5株,agrⅡ型2株,agrⅢ型2株,agr未分型11株。64株为MRSE,占76.20%,其中agrⅠ型37株,agrⅡ型7株,agrⅢ型11株,agr未分型9株。血液来源的表皮葡萄球菌中,agrⅠ型25株,agrⅡ型5株,agrⅢ型10株,agr未分型16株。非血液来源的表皮葡萄球菌中,agrⅠ型17株,agrⅡ型4株,agrⅢ型3株,agr未分型4株。结论临床分离表皮葡萄球菌agr基因存在明显多态性,MSSE中未分型agr较常见,MRSE中以agrⅠ型和agrⅢ型为主。
[Abstract]:Objective to investigate the polymorphism of agr gene of Staphylococcus epidermidis (Staphylococcus epidermidis) and to understand the molecular characteristics of Staphylococcus epidermidis. Methods 84 strains of Staphylococcus epidermidis were collected and identified by automatic microbial identification system. Esp and mecA genes were amplified by PCR to identify and distinguish methicillin-sensitive Staphylococcus epidermidis from methicillin-resistant Staphylococcus epidermidis. The agr allele of Staphylococcus epidermidis was amplified by multiple PCR, and the polymorphism of agr gene was analyzed. Results among 84 clinical isolates of Staphylococcus epidermidis, 20 strains were MSSEs, accounting for 23.80 strains. Among them, 5 strains of agr type 鈪，

本文编号：1801553