全反式维甲酸对肺气肿模型大鼠的干预作用

发布时间：2018-04-26 08:57

本文选题：肺气肿 + 大鼠　；参考：《中国新药与临床杂志》2010年02期

【摘要】：目的研究不同时间段给予全反式维甲酸(ATRA)对肺气肿模型大鼠的干预作用及其机制。方法60只SD大鼠随机分为6组:生理盐水组(N组)、模型组(P组)、棉籽油组(C组)、早期干预组(R1组)、中期干预组(R2组)和后期干预组(R3组),每组10只。N组大鼠气管滴注生理盐水1mL·kg-1,其余5组气管滴注5%木瓜蛋白酶1mL·kg-1建立肺气肿模型。R1、R2和R3组分别于造模后15～30d、30～45d和45～60d给予ATRA500μg·kg-1腹腔注射,C组给予棉籽油腹腔注射。观察各组支气管肺泡灌洗液(BALF)中血管内皮生长因子(VEGF)含量和细胞数,肺组织病理学改变及肺组织血管内皮生长因子受体2(VEGFR-2)、基质金属蛋白酶1(MMP-1)的表达水平。结果与N组比较,P组BALF中细胞总数明显升高(P0.01),其中以巨噬细胞和中性粒细胞为主。R1、R2和R3组与P组比较细胞总数降低(P0.01),此3组之间无显著差异。与N组比较,其余5组平均肺泡面积和内衬间隔增大,每个视野内肺泡数减少(P0.01);与P组相比,R1、R2和R3组肺泡数增加、平均肺泡面积减小(P0.01),以R1组最为明显。与N组相比,P组VEGF和VEGFR-2表达降低,MMP-1表达增高(P0.01);与P组相比,R1、R2和R3组VEGF和VEGFR-2表达增高,MMP-1表达降低(P0.05),R1、R2和R3组之间无显著差异。结论ATRA可以促进肺泡再生,早期干预效果较佳,这种作用可能与调节VEGF、VEGFR-2、MMP-1有关。
[Abstract]:Objective to study the effects of ATRA-ATRA-ATRA-ATRA-ATRA-ATRA-ATRA-ATRA-ATRA-induced emphysema in rats with emphysema. Methods Sixty Sprague-Dawley rats were randomly divided into 6 groups: normal saline group (n group), model group (P group), cottonseed oil group (group C), early intervention group (group R1), intermediate intervention group (group R 2) and late intervention group (group C) with 10 rats in each group. The emphysema model was established by instilling 5% papain 1mL kg-1 into the trachea of the other five groups. The emphysema model was established in group R1 and group R3. The emphysema model was established by intraperitoneal injection of ATRA500 渭 g kg-1 for 45 days and 45 days after injection of ATRA500 渭 g kg-1 for 45 days and 4560 days after the establishment of the model, and the other five groups were given cottonseed oil intraperitoneally. The content and cell number of vascular endothelial growth factor (VEGF) in bronchoalveolar lavage fluid (BALF), lung histopathological changes and the expression of vascular endothelial growth factor receptor 2 (VEGFR-2) and matrix metalloproteinase-1 (MMP-1) were observed. Results compared with group N, the total number of cells in BALF of P group was significantly higher than that of group N, and the total number of cells in group P was significantly lower than that in group P (P 0.01), especially in macrophages and neutrophils, especially in the macrophages and neutrophils, and the total number of cells in group R3 was lower than that in group P, but there was no significant difference among the three groups. Compared with group N, the mean alveolar area and lining interval in the other five groups were increased, the number of alveoli in each field of vision was decreased (P 0.01), the number of alveoli in group R 1 and group R 3 was increased, and the mean alveolar area was decreased in group R 1, especially in group R 1. Compared with group N, the expression of VEGF and VEGFR-2 in P group was decreased and the expression of MMP-1 was increased. There was no significant difference in the expression of VEGF and VEGFR-2 between P group and P group (P 0 05) and group R 3. There was no significant difference between group R 2 and group R 3. Conclusion ATRA can promote alveolar regeneration, and the early intervention effect may be related to the regulation of VEGFR-2 and MMP-1.
【作者单位】：安徽医科大学第一附属医院老年呼吸内科;合肥市第一人民医院;
【基金】：安徽省教育厅自然科学基金资助项目(KJ2009A118)
【分类号】：R563.3

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