Ghrelin对食源性肥胖小鼠血管生成标志物的影响研究

发布时间：2018-04-27 22:23

本文选题：Ghrelin + 小鼠　；参考：《青岛大学》2013年硕士论文

【摘要】：目的：Ghrelin是一种由胃肠道分泌的脑肠肽,其是生长激素促分泌素受体的内源性配体；然而最近研究发现,Ghrelin对心血管也有影响。本研究观察ghrelin对食源性肥胖小鼠血清血管生成标志物瘦素、一氧化氮(N0)、血管内皮细胞生长因子(VEGF)及其可溶性受体(VEGF-1和sFlt-1)表达的影响。方法：雄性小鼠随机分为四组,普通饮食组(ND), ghrelin+ND组,高脂饮食组(HFD),ghrelin+HFD组(每组6只小鼠)。HFD组和ND组小鼠分别给予高脂饮食或正常饮食15周,100μg/kg的剂量皮下注射ghrelin,每日两次持续10天。实验结束后抽血检测血糖、血清胰岛素、VEGF、sFlt-1、NO以及瘦素的含量。结果：高脂饮食组小鼠与正常饮食组小鼠相比,血清N0浓度以及瘦素浓度显著升高(t=4.08, P0.01; t=12.49, P0.01),但血清VEGF浓度及sFlt-1水平没有明显变化(t=0.98, P0.05; t=1.75, P0.05)。注射ghrelin可使高脂饮食组小鼠血清VEGF浓度显著升高(t=3.94,P0.01),而血清瘦素(t=3.51,P0.01)以及N0浓度显著下降(t=3.25,P0.01)。结论：Ghrelin可改变血清中与血管生成有关生物标记物表达。
[Abstract]:Aim: ghrelin is a brain gut peptide secreted by the gastrointestinal tract, which is an endogenous ligand of growth hormone secretin receptor. However, recent studies have found that ghrelin also has cardiovascular effects. The aim of this study was to investigate the effects of ghrelin on the expression of serum angiogenic markers leptin, nitric oxide (N0), vascular endothelial growth factor (VEGF) and its soluble receptors (VEGF-1 and sFlt-1) in food-borne obese mice. Methods: male mice were randomly divided into four groups: normal diet group, ghrelin ND group. High fat diet group (6 mice in each group) and ND group (n = 6) were subcutaneously injected with ghrelin at the dose of 100 渭 g/kg for 15 weeks after high fat diet or normal diet, twice a day for 10 days. At the end of the experiment, blood glucose, serum insulin level and leptin were measured. Results: compared with the normal diet group, the serum N0 and leptin concentrations in the high-fat diet group were significantly higher than those in the normal diet group (4.08, P 0.01; t _ (12.49), P 0.01), but there were no significant changes in the serum VEGF concentration and sFlt-1 levels in the control group (0.98, P 0.05; t _ (1.75), P 0.05). The concentration of serum VEGF in high-fat diet group was significantly higher than that in high-fat diet group, while the concentration of serum leptin was 3.51g / P0.01) and the concentration of N0 was significantly lower than that of control group (3.25g / P0.01g / kg). ConclusionTwo one ghrelin can alter the expression of angiogenic biomarkers in serum.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R363

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