蜡螟在念珠菌属真菌动物模型中应用价值的探究

发布时间：2018-04-28 12:23

本文选题：真菌 + 蜡螟　；参考：《中国微生态学杂志》2015年06期

【摘要】：目的构建蜡螟的念珠菌属真菌动物模型并探究真菌对蜡螟的细胞、组织、器官的影响以及念珠菌属真菌蜡螟模型的应用价值。方法用注射器吸取一定量的菌液注入蜡螟体内,在30℃培养箱中培养一定时间后,取出其中的一小部分蜡螟进行解剖,观察真菌菌丝对蜡螟肠道组织的损伤情况,取另一小部分蜡螟做病理切片,同时记录不同时间段蜡螟的生存死亡情况;将空白蜡螟断头取血,分离淋巴细胞后用DMEM培养,用热灭活真菌处理蜡螟淋巴血细胞,37℃培养3 h后,分别用ROS(氧化应激水平)探针和溶酶体探针标记ROS、溶酶体,观察真菌刺激细胞时的自噬水平的变化。结果真菌菌丝对蜡螟的肠道组织产生损伤,病理切片显示大量菌丝生长,细胞吞噬真菌后,ROS升高,溶酶体与菌共定位。结论念珠菌属真菌的蜡螟模型中,真菌对蜡螟的细胞、组织、器官均有损伤,蜡螟的细胞自噬水平升高,并且此模型具有易操作,价格低廉,便于进行统计学分析等优势。
[Abstract]:Objective to construct the Candida albicans model of the wax borer and explore the effect of fungi on the cells, tissues and organs of the wax borer, and the application value of the Candida albicans model of the wax borer. Methods a certain amount of liquid was injected into the body of the wax borer, and a small portion of the wax borer was taken out after a fixed time in the incubator at 30. The damage of the intestinal tissue of the fungus mycelia to the intestinal tissue of the wax borer was observed, and another small part of the wax borer was taken as a pathological section. At the same time, the survival and death of the wax borer were recorded at the same time. The blood of the blank wax borer was taken from the head. The lymphocyte was cultured with DMEM and the heat inactivated fungus was used to treat the lymphoblastic blood cells of the wax borer. After 3 h culture at 37 C, ROS was used respectively. (oxidative stress level) probe and lysosome probe labeled ROS, lysosome, observed the changes in the autophagy level during the fungal stimulation of the cells. Results fungal mycelium damage to the intestinal tissue of the wax borer, pathological sections showed a large number of mycelium growth, after the cells phagocyted fungi, ROS increased, lysosomes and bacteria Co located. Conclusion the wax borer of Candida fungi In the model, the fungi have damage to the cells, tissues and organs of the wax borer. The autophagy level of the cells of the wax borer is increased, and the model has the advantages of easy operation, low price, and convenient for statistical analysis.

【作者单位】：安徽理工大学医学院免疫与检验教研室;免疫与感染研究所;大连医科大学中西医结合研究院;大连医科大学附属第二医院;
【基金】：辽宁省科技厅项目(2012225020)
【分类号】：R519;R-332

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