原位逆行灌注兔肾IRI模型的建立及术后肾功能与组织结构的研究

发布时间：2018-04-29 10:09

本文选题：兔肾 + 缺血再灌注　；参考：《中国医学工程》2014年08期

【摘要】：目的观察原位逆行灌注法对兔子肾脏缺血再灌注损伤的保护,为临床提供理论依据。方法 20只新西兰雄性大白兔,随机分为二组,顺行灌注组与逆行灌注组,每组10只,在建立缺血再灌注损伤(IRI)模型后,二组分别于术前、术后第1、2、3、5、7天采静脉血,分别检测血清肌酐(SCr)、尿素氮(BUN),第7天取标本做常规病理及电镜检查。最后统一测定每组兔子第1天的IL-1浓度值。结果顺行灌注组SCr、BUN值升高的快,与逆行灌注组相比有显著差异(P0.01)。肾脏组织病理与电镜肾小管超微结构的损伤程度与SCr、BUN呈正相关。顺行灌注组1 d时IL-1浓度高,和逆行灌注组相比差异有统计学意义(P0.01)。结论与顺行灌注组相比逆行灌注组对兔子肾IRI更具有保护作用。逆行灌注法可能为肾移植带来更为安全、便捷的手段。
[Abstract]:Objective to observe the protective effect of in situ retrograde perfusion on renal ischemia reperfusion injury in rabbits. Methods Twenty New Zealand male white rabbits were randomly divided into two groups: antegrade perfusion group (n = 10) and retrograde perfusion group (n = 10). Serum creatinine (SCR) and urea nitrogen (bun) were detected respectively. The specimens were taken for routine pathological and electron microscopic examination on the 7th day. The IL-1 concentration on the first day of each group was determined uniformly. Results compared with the retrograde perfusion group, the value of SCrnbun in the antegrade perfusion group increased rapidly (P 0.01). The degree of injury of renal histopathology and ultrastructure of renal tubules was positively correlated with SCrBUN. The concentration of IL-1 in the antegrade perfusion group was higher than that in the retrograde perfusion group on day 1, and the difference was statistically significant compared with that in the retrograde perfusion group (P 0.01). Conclusion compared with the anterograde perfusion group, the retrograde perfusion group has more protective effect on renal IRI in rabbits. Retrograde perfusion may be a safe and convenient method for renal transplantation.
【作者单位】：乌兰察布市中心医院泌尿外科;昆明医科大学第一附属医院移植中心;
【分类号】：R-332;R699.2

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