小鼠神经干细胞免疫原性的研究

发布时间：2018-04-29 20:16

  本文选题：神经干细胞 + 免疫原性　； 参考：《细胞与分子免疫学杂志》2010年04期

【摘要】：目的:探讨小鼠神经干细胞(NSCs)免疫原性,以利于解决神经干细胞移植中的免疫排斥问题。方法:(1)首先将30只C57BL/6近交系小鼠随机分成两组,以BALB/c近交系小鼠的NSCs及肝细胞(作为体细胞对照组)分别进行腹腔主动免疫,然后将NSCs及肝细胞分别与各自免疫后小鼠的T淋巴细胞进行单向混合淋巴细胞培养,通过液闪计数仪检测各自T淋巴细胞增殖程度,从而比较两者免疫原性的强弱。(2)利用异硫氰酸荧光素标记抗体(FITC)及Phycoery-thrin(PE)抗体分别标记BALB/c小鼠NSCs主要组织相容性抗原复合物Ⅰ、Ⅱ(MHC-Ⅰ、MHC-Ⅱ),然后通过流式细胞术(FCM)分别检测BALB/c小鼠NSCs及其肝细胞的MHC-Ⅰ和MHC-Ⅱ阳性细胞比例,从而推断小鼠NSCs的免疫原性。结果:(1)NSC组液闪计数仪检测所得cpm值为16592.8±2865.3,肝细胞组为27815.0±2416.3,NSC组明显低于肝细胞组,差别有统计学意义(P0.01)。(2)小鼠NSCs实验组中,约(87.55±3.65)%的增殖期神经干细胞没有检测到MHC-Ⅰ和MHC-Ⅱ分子表达;而作为对照组的小鼠肝细胞组中,仅有约(27.45±1.86)%的肝细胞未表达MHC-Ⅰ和MHC-Ⅱ分子。且NSC组MHC-Ⅰ和MHC-Ⅱ阴性细胞比例明显高于肝细胞组,差别有统计学意义(P0.01)。结论:小鼠神经干细胞具有较弱的免疫原性,且其免疫原性明显低于同一个体的肝细胞。
[Abstract]:Aim: to investigate the immunogenicity of neural stem cells (NSCs) in mice in order to solve the problem of immune rejection in neural stem cell transplantation. Methods at first, 30 C57BL/6 inbred mice were randomly divided into two groups. The NSCs of BALB/c inbred mice and hepatocytes (as control group) were immunized by intraperitoneal active immunization. Then NSCs and hepatocytes were cultured with the T lymphocytes of mice immunized with each other, and the proliferation degree of T lymphocytes was measured by liquid scintillation counter. Therefore, the immunogenicity of the two groups was compared. (2) using fluorescein isothiocyanate (FITC) antibody and Phycoery-thrin (PEE) antibody to label the main histocompatibility antigen complex 鈪，

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